In Vitro Micropropagation of the Medicinal Plant Physalis angulata L
| Autor: | Sape Subba Tata, Owk Aniel Kumar, Songa Ramesh |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Medicine (miscellaneous)
Physalis angulata Plant Science Horticulture Biochemistry Genetics and Molecular Biology (miscellaneous) chemistry.chemical_compound Murashige and Skoog medium Botany lcsh:Agriculture (General) lcsh:Science (General) Gibberellic acid biology fungi food and beverages Forestry biology.organism_classification lcsh:S1-972 Agricultural and Biological Sciences (miscellaneous) In vitro chemistry Micropropagation Shoot Medicinal herbs Agronomy and Crop Science lcsh:Q1-390 Explant culture |
| Zdroj: | Notulae Scientia Biologicae, Vol 8, Iss 2, Pp 161-163 (2016) |
| ISSN: | 2067-3264 2067-3205 |
| DOI: | 10.15835/nsb829817 |
| Popis: | Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L. |
| Databáze: | OpenAIRE |
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