Analysis of the effects of CPA and L-histidine on goldfish tested on a conditioned place preference model
| Autor: | C.C. Medalha, J. L. Coelho, Rosana Mattioli |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Chlorpheniramine
Motivation Dose-Response Relationship Drug Conditioning Classical Histaminergic Antagonist Histamine h Choice Behavior Conditioned place preference Behavioral Neuroscience chemistry.chemical_compound Animal science chemistry Goldfish Orientation Carassius auratus Avoidance Learning Histamine H1 Antagonists Animals Histidine Habituation Histamine Injections Intraperitoneal |
| Zdroj: | Behavioural brain research. 124(2) |
| ISSN: | 0166-4328 |
| Popis: | The aim of this study was to investigate whether the histamine H 1 - receptor blocker chlorpheniramine (CPA) and the histaminergic precursor l -histidine have reinforcing effects in goldfish ( Carassius auratus ). We used a place-preference procedure in a four-compartment aquarium in which, on the first day, the animals were placed for 30 min for habituation. On the second day, the time spent in each chamber was recorded for 10 min in order to determine which was preferred. After 24 h a non-transparent acrylic division was placed in the aquarium to make four identical compartments. The fish were injected intraperotoneally (i.p.) with l -histidine in three doses: 100, 250, and 500 mg/kg of body-weight ( n =20) and with vehicle control ( n =20). After 30 min the animals were placed in the intermediate preference chamber for another 30 min. The CPA was injected i.p in four doses; 0.4, 1.0, 4.0 or 8.0 mg/kg ( n =16) or vehicle ( n =16) and after 5 min the animals were placed in the intermediate preference chamber for another 30 min. On the fourth day the procedure of the second day was repeated. The results indicate that the group treated with 500 mg/kg l -histidine spent significantly more time in the paired compartment when compared with the time spent before treatment (Wilcoxon Signed Rank Test, P =0.0172). The same behavior was not observed in other treatment groups ( P >0.05). The results indicate a reinforcing effect of l -histidine in this place preference model with goldfish. |
| Databáze: | OpenAIRE |
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