Upregulation of intracellular antioxidant enzymes in brain and heart during estivation in the African lungfish Protopterus dolloi
| Autor: | Jeffrey A. Stuart, Sarah A. Warren, Shit F. Chew, James S. Ballantyne, Kurtis D. Salway, Yuen K. Ip, Melissa M. Page |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Physiology African lungfish Glutathione reductase Biology medicine.disease_cause Biochemistry Protein Carbonylation Superoxide dismutase Endocrinology Protopterus dolloi Internal medicine medicine Animals Potassium Cyanide Ecology Evolution Behavior and Systematics Lungfish chemistry.chemical_classification Aldehydes Glutathione Peroxidase Superoxide Dismutase Myocardium Glutathione peroxidase Fishes Brain Proteins Fasting Catalase biology.organism_classification Estivation Up-Regulation Glutathione Reductase chemistry Biocatalysis biology.protein Aestivation Tyrosine Animal Science and Zoology Oxidoreductases Oxidative stress |
| Zdroj: | Journal of Comparative Physiology B. 180:361-369 |
| ISSN: | 1432-136X 0174-1578 |
| DOI: | 10.1007/s00360-009-0416-7 |
| Popis: | The African slender lungfish, Protopterus dolloi, is highly adapted to withstand periods of drought by secreting a mucous cocoon and estivating for periods of months to years. Estivation is similar to the diapause and hibernation of other animal species in that it is characterized by negligible activity and a profoundly depressed metabolic rate. As is typically observed in quiescent states, estivating P. dolloi are resistant to environmental stresses. We tested the hypothesis that P. dolloi enhances stress resistance during estivation by upregulating intracellular antioxidant defences in brain and heart tissues. We found that most of the major intracellular antioxidant enzymes, including the mitochondrial superoxide dismutase, cytosolic superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, were upregulated in brain tissue of lungfish that had estivated for 60 days. Several of these enzymes were also elevated in heart tissue of estivators. These changes were not due to food deprivation, as they did not occur in a group of fish that were deprived of food but maintained in water for the same period of time. We found little evidence of tissue oxidative damage in estivators. Products of lipid peroxidation (4-hydroxynonenal adducts) and oxidative protein damage (carbonylation) were similar in estivating and control lungfish. However, protein nitrotyrosine levels were elevated in brain tissue of estivators. Taken together, these data indicate that estivating P. dolloi have enhanced oxidative stress resistance in brain and heart due to a significant upregulation of intracellular antioxidant capacity. |
| Databáze: | OpenAIRE |
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