LED-enhanced biosynthesis of biologically active ingredients in callus cultures of Ocimum basilicum
| Autor: | Waqar Ahmad, Adnan Zahir, Bilal Haider Abbasi, Muhammad Nadeem, Christophe Hano, Muhammad Zeeshan Younas |
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| Přispěvatelé: | Department of Biotechnology, University of Tehran-University College of Science, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), Université Francois Rabelais [Tours] |
| Rok vydání: | 2019 |
| Předmět: |
Ocimum bassilicum
rosmarinic acid [SDV.BIO]Life Sciences [q-bio]/Biotechnology food.ingredient Light LEDs Phytochemicals 030303 biophysics Cyanidin Flavonoid Cell Culture Techniques Biophysics Color 02 engineering and technology Depsides Antioxidants 03 medical and health sciences chemistry.chemical_compound food Phenols Radiology Nuclear Medicine and imaging Biomass Food science Melatonin chemistry.chemical_classification 0303 health sciences Peonidin Plants Medicinal Radiation Radiological and Ultrasound Technology biology Rosmarinic acid Basilicum 021001 nanoscience & nanotechnology Ocimum biology.organism_classification anthocyanins Eugenol chemistry Cinnamates Callus flavonoids Ocimum basilicum phenolic 0210 nano-technology eugenol |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology Journal of Photochemistry and Photobiology B: Biology, Elsevier, 2019, 190, pp.172-178. ⟨10.1016/j.jphotobiol.2018.09.011⟩ |
| ISSN: | 1011-1344 |
| Popis: | International audience; Ocimum basilicum is a medicinal plant with multiple health benefits including cardiovascular, cancer and diabetics. In the present study, the influences of light emitting diodes (LEDs) were investigated on the accumulation of biologically active ingredients in callus cultures of Ocimum basilicum. Among the various tested treatments optimum levels of Total phenolic content (TPC) was noted in callus culture grown under blue lights as compared to control, while maximum accumulation of Total flavonoid content (TFC) was noted in callus culture grown under red light as compared to control. HPLC analyses showed that highest concentrations of Rosmarinic acid (96.0 mg/g DW) and Eugenol (0.273 mg/g DW) were accumulated in blue light which was 2.46 and 2.25 times greater than control (39.0 mg/g DW, 0.171 mg/g DW), respectively. Chicoric acid (81.40 mg/g DW) optimum accumulation was noted in callus grown under the continuous white light, which was almost 4.52 times greater than control. Anthocyanins content were also analyzed, the highest amount of Peonidin (0.127 mg/g DW) and cyanidin (0.1216 mg/g DW) were found in callus culture grown under red light. These findings suggest that application of LED's is a promising strategy for enhancing production of biologically active ingredients in callus cultures Ocimum basilicum. |
| Databáze: | OpenAIRE |
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