Development and characterization of rhVEGF-loaded poly(HEMA–MOEP) coatings electrosynthesized on titanium to enhance bone mineralization and angiogenesis
| Autor: | Antonio Zizzi, S Manzotti, Stefania Cometa, Elvira De Giglio, D. Cafagna, Maria Antonietta Ricci, Luigia Sabbatini, Monica Mattioli-Belmonte |
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| Rok vydání: | 2010 |
| Předmět: |
Vascular Endothelial Growth Factor A
Materials science Cell Survival Simulated body fluid Biomedical Engineering Neovascularization Physiologic Tetrazolium Salts chemistry.chemical_element Biocompatible Materials Nanotechnology Methacrylate Biochemistry Mineralization (biology) Osseointegration Biomaterials chemistry.chemical_compound Calcification Physiologic Cell Line Tumor Humans Molecular Biology Titanium Osteoblasts technology industry and agriculture Phosphorus General Medicine Phosphate Recombinant Proteins Thiazoles Chemical engineering chemistry Self-healing hydrogels Methacrylates Calcium Implant Biotechnology |
| Zdroj: | Acta Biomaterialia. 6:282-290 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2009.07.008 |
| Popis: | Osteointegration of titanium implants could be significantly improved by coatings capable of promoting both mineralization and angiogenesis. In the present study, a copolymeric hydrogel coating, poly-2-hydroxyethyl methacrylate-2-methacryloyloxyethyl phosphate (P(HEMA-MOEP)), devised to enhance calcification in body fluids and to entrap and release growth factors, was electrosynthesized for the first time on titanium substrates and compared to poly-2-hydroxyethyl methacrylate (PHEMA), used as a blank reference. Polymers exhibiting negatively charged groups, such as P(HEMA-MOEP), help to enhance implant calcification. The electrosynthesized coatings were characterized by X-ray photoelectron spectroscopy and atomic force microscopy. MG-63 human osteoblast-like cell behaviour on the coated specimens was investigated by scanning electron microscopy, MTT viability test and osteocalcin mRNA detection. The ability of negatively charged phosphate groups to promote hydroxyapatite-like calcium phosphate deposition on the implants was explored by immersing them in simulated body fluid. Similar biological responses were observed in both coated specimens, while calcium-phosphorus globules were detected only on P(HEMA-MOEP) surfaces pretreated with alkaline solution. Testing of the ability of P(HEMA-MOEP) hydrogels to entrap and release human recombinant vascular endothelial growth factor, to tackle the problem of insufficient oxygen and nutrient delivery, suggested that P(HEMA-MOEP)-coated titanium prostheses could represent a multifunctional material suitable for bone restoration applications. |
| Databáze: | OpenAIRE |
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