Contribution of DNA polymerase eta to immunoglobulin gene hypermutation in the mouse
| Autor: | Jean-Claude Weill, Annie De Smet, Said Aoufouchi, Ahmad Faili, Claude-Agnès Reynaud, Frédéric Delbos |
|---|---|
| Přispěvatelé: | Développement du Systeme Immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ministère de la Recherche (A.C.I. Biologie du Développement et Physiologie Intégrative), Ligue Nationale Contre le Cancer, Fondation Princesse Grace de Monaco, Delbos, Frédéric, Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
DNA Repair
DNA polymerase Base Pair Mismatch DNA-Directed DNA Polymerase MESH: Base Sequence MESH: Mice Knockout Mice Peyer's Patches 0302 clinical medicine MESH: DNA-Directed DNA Polymerase Immunology and Allergy MESH: Animals Polymerase Genetics Mice Knockout MESH: DNA Repair 0303 health sciences B-Lymphocytes biology Genes Immunoglobulin somatic hypermutation immunoglobulin genes DNA Polymerase iota polymerase eta [SDV.IMM]Life Sciences [q-bio]/Immunology Immunoglobulin gene congenital hereditary and neonatal diseases and abnormalities MESH: Somatic Hypermutation Immunoglobulin [SDV.IMM] Life Sciences [q-bio]/Immunology Mismatch repair complex DNA repair DNA polymerase II Immunology Molecular Sequence Data Somatic hypermutation 03 medical and health sciences MESH: B-Lymphocytes Animals MESH: Mice 030304 developmental biology MESH: Base Pair Mismatch MESH: Molecular Sequence Data Base Sequence Mutagenesis Brief Definitive Report nutritional and metabolic diseases Molecular biology MESH: Genes Immunoglobulin biology.protein MESH: Peyer's Patches Somatic Hypermutation Immunoglobulin 030215 immunology |
| Zdroj: | Journal of Experimental Medicine Journal of Experimental Medicine, 2005, 201 (8), pp.1191-6. ⟨10.1084/jem.20050292⟩ Journal of Experimental Medicine, Rockefeller University Press, 2005, 201 (8), pp.1191-6. ⟨10.1084/jem.20050292⟩ The Journal of Experimental Medicine The Journal of Experimental Medecine The Journal of Experimental Medecine, The Rockefeller University Press, 2005, 201 (8), pp.1191-6. 〈10.1084/jem.20050292〉 |
| ISSN: | 0022-1007 1540-9538 |
| DOI: | 10.1084/jem.20050292⟩ |
| Popis: | International audience; The mutation pattern of immunoglobulin genes was studied in mice deficient for DNA polymerase eta, a translesional polymerase whose inactivation is responsible for the xeroderma pigmentosum variant (XP-V) syndrome in humans. Mutations show an 85% G/C biased pattern, similar to that reported for XP-V patients. Breeding these mice with animals harboring the stop codon mutation of the 129/Olain background in their DNA polymerase iota gene did not alter this pattern further. Although this G/C biased mutation profile resembles that of mice deficient in the MSH2 or MSH6 components of the mismatch repair complex, the residual A/T mutagenesis of pol eta-deficient mice differs markedly. This suggests that, in the absence of pol eta, the MSH2-MSH6 complex is able to recruit another DNA polymerase that is more accurate at copying A/T bases, possibly pol kappa, to assume its function in hypermutation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|