Channel catfish (Ictalurus punctatus Rafinesque, 1818) tetraspanin membrane protein family: Identification, characterization and expression analysis of CD63 cDNA
Autor: | Phillip H. Klesius, Hung-Yueh Yeh |
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Rok vydání: | 2010 |
Předmět: |
DNA
Complementary animal structures Molecular Sequence Data Immunology Gene Expression Platelet Membrane Glycoproteins Biology Tetraspanin Antigens CD Complementary DNA Animals Tissue Distribution Amino Acid Sequence RNA Messenger Integral membrane protein Conserved Sequence Phylogeny DNA Primers Base Sequence Sequence Homology Amino Acid General Veterinary Tetraspanin 30 fungi biology.organism_classification Molecular biology Transmembrane protein Protein Structure Tertiary Cell biology Ictaluridae Transmembrane domain Membrane protein Ictalurus Catfish |
Zdroj: | Veterinary Immunology and Immunopathology. 133:302-308 |
ISSN: | 0165-2427 |
Popis: | CD63, known as lysosome associated membrane protein 3 (LAMP-3), is a member of the tetraspanin integral membrane protein family. This protein plays many important roles in immuno-physiological functions. In this communication, we report the identification, characterization, and expression analysis of the channel catfish CD63 transcript. The complete nucleic acid sequence of channel catfish CD63 cDNA was comprised of 1159 nucleotides, including an open reading frame, which appears to encode a putative peptide of 237-amino-acid residues. Like other tetraspanin proteins, the channel catfish CD63 peptide can be divided into domains, including four transmembrane domains, three intracellular domains, and one of each small and large extracellular loops. The channel catfish CD63 peptide shares 52-55% identity among fish counterparts, but only 43-46% identity among mammalian counterparts. The characteristic Cys-Cys-Gly motif and four Cys residues in the large extracellular loop were conserved. The channel catfish CD63 transcript was detected by RT-PCR in spleen, anterior kidney, liver, intestine, skin and gill. This result provides important information for further elucidating CD63 functions in channel catfish. |
Databáze: | OpenAIRE |
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