Identification of amygdala-expressed genes associated with autism spectrum disorder

Autor: Arnold R. Kriegstein, Maria Jesus Herrero, Catherine Sullivan, David Hernandez-Pineda, Shawn F. Sorrells, Payal Banerjee, Saarthak Sethi, Dmitry Velmeshev, Abha R. Gupta, Joshua G. Corbin
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Autism Spectrum Disorder
Autism
Gene Expression
lcsh:RC346-429
Mice
0302 clinical medicine
Gene expression
Databases
Genetic
2.1 Biological and endogenous factors
Gene Regulatory Networks
Autism spectrum disorder
Pediatric
0303 health sciences
Single nucleus RNA sequencing
Brain
Amygdala
Phenotype
Brain development
Psychiatry and Mental health
Mental Health
medicine.anatomical_structure
ASD genes
Disease Susceptibility
Biotechnology
Signal Transduction
Pediatric Research Initiative
Cell type
Intellectual and Developmental Disabilities (IDD)
Clinical Sciences
Biology
behavioral disciplines and activities
Databases
03 medical and health sciences
Genetic
Developmental Neuroscience
Behavioral and Social Science
mental disorders
Genetics
medicine
Animals
Humans
Genetic Predisposition to Disease
Molecular Biology
Gene
Alleles
lcsh:Neurology. Diseases of the nervous system
030304 developmental biology
Research
Gene Expression Profiling
Human Genome
Neurosciences
Computational Biology
medicine.disease
Human genetics
Brain Disorders
Gene Ontology
Transcriptome
Neuroscience
030217 neurology & neurosurgery
Biomarkers
Developmental Biology
Social behavior
Zdroj: Molecular Autism, Vol 11, Iss 1, Pp 1-14 (2020)
Molecular Autism
Molecular autism, vol 11, iss 1
ISSN: 2040-2392
Popis: Background Studies of individuals with autism spectrum disorder (ASD) have revealed a strong multigenic basis with the identification of hundreds of ASD susceptibility genes. ASD is characterized by social deficits and a range of other phenotypes, implicating complex genetics and involvement of a variety of brain regions. However, how mutations and mis-expression of select gene sets are associated with the behavioral components of ASD remains unknown. We reasoned that for genes to be associated with ASD core behaviors they must be: (1) expressed in brain regions relevant to ASD social behaviors and (2) expressed during the ASD susceptible window of brain development. Methods Focusing on the amygdala, a brain region whose dysfunction has been highly implicated in the social component of ASD, we mined publicly available gene expression databases to identify ASD-susceptibility genes expressed during human and mouse amygdala development. We found that a large cohort of known ASD susceptibility genes is expressed in the developing human and mouse amygdala. We further performed analysis of single-nucleus RNA-seq (snRNA-seq) data from microdissected amygdala tissue from five ASD and five control human postmortem brains ranging in age from 4 to 20 years to elucidate cell type specificity of amygdala-expressed genes and their dysregulation in ASD. Results Our analyses revealed that of the high-ranking ASD susceptibility genes, 80 are expressed in both human and mouse amygdala during fetal to early postnatal stages of development. Our human snRNA-seq analyses revealed cohorts of genes with altered expression in the ASD amygdala postnatally, especially within excitatory neurons, with dysregulated expression of seven genes predicted from our datamining pipeline. Limitations We were limited by the ages for which we were able to obtain human tissue; therefore, the results from our datamining pipeline approach will require validation, to the extent possible, in human tissue from earlier developmental stages. Conclusions Our pipeline narrows down the number of amygdala-expressed genes possibly involved in the social pathophysiology of ASD. Our human single-nucleus gene expression analyses revealed that ASD is characterized by changes in gene expression in specific cell types in the early postnatal amygdala.
Databáze: OpenAIRE
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