A herpesvirus antigen in human premalignant and malignant cervical biopsies and explants
| Autor: | L. Mercer, C.K. Lumpkin, Dean R. Goplerud, F. Marciano-Cabral, G.A. Cabral, D. Fry |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Pathology
medicine.medical_specialty Biopsy Cytological Techniques Uterine Cervical Neoplasms Cervix Uteri Biology medicine.disease_cause Virus Immunoenzyme Techniques Antigen medicine Humans Simplexvirus Antigens Viral Tumor Antigens Viral Cells Cultured medicine.diagnostic_test Carcinoma in situ Obstetrics and Gynecology Uterine Cervical Dysplasia medicine.disease Squamous metaplasia Uterine Cervicitis Microscopy Electron Herpes simplex virus Dysplasia Cell culture Carcinoma Squamous Cell Female Carcinoma in Situ |
| Zdroj: | American Journal of Obstetrics and Gynecology. 145:79-86 |
| ISSN: | 0002-9378 |
| Popis: | Cervical biopsies and explant cultures from patients with squamous metaplasia, various grades of dysplasia, carcinoma in situ (CIS), and invasive squamous cell carcinoma were screened for VP143, an early nonstructural polypeptide of herpes simplex virus type 2 (HSV-2), VP143 was identified in 31% of biopsies exhibiting severe dysplasia, 29% with CIS, and 41% with invasive squamous cell carcinoma. Similar results were obtained when explants derived from these biopsies were examined for VP 143. The expression of the protein persisted in passaged subcultures in four of five invasive carcinomas which originally contained VP 143. Staining for VP 157, the major capsid protein of HSV-2, was absent. Furthermore, virus structures were not seen by electron microscopy and infectious virus was not isolated from cell cultures inoculated with biopsy extracts. These results suggest that VP 143 was expressed in the premalignant amd malignant cervical cells in the absence of productive viral infection. Thus, a fragment of the HSV02 genome was retained within the cells, the expression of which resulted in the production of VP 143. |
| Databáze: | OpenAIRE |
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