Ancient Gene Duplication Provided a Key Molecular Step for Anaerobic Growth of Baker's Yeast
| Autor: | Masaya Hayashi, Elizabeth A. Craig, Barry L. Williams, Brenda Schilke, Jaroslaw Marszalek |
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| Rok vydání: | 2011 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Blotting Western Molecular Sequence Data Saccharomyces cerevisiae Biology Evolution Molecular Gene Duplication Mitochondrial Precursor Protein Import Complex Proteins Gene duplication Genetics Amino Acid Sequence Anaerobiosis Saccharomycotina Molecular Biology Gene Phylogeny Research Articles Ecology Evolution Behavior and Systematics Membrane Proteins Membrane Transport Proteins biology.organism_classification Aerobiosis Transmembrane protein Mitochondria Transport protein Cell biology Protein Transport Membrane protein Electrophoresis Polyacrylamide Gel Intermembrane space Sequence Alignment |
| Zdroj: | Molecular Biology and Evolution. 28:2005-2017 |
| ISSN: | 1537-1719 0737-4038 |
| Popis: | Mitochondria are essential organelles required for a number of key cellular processes. As most mitochondrial proteins are nuclear encoded, their efficient translocation into the organelle is critical. Transport of proteins across the inner membrane is driven by a multicomponent, matrix-localized ‘‘import motor,’’ which is based on the activity of the molecular chaperone Hsp70 and a J-protein cochaperone. In Saccharomyces cerevisiae, two paralogous J-proteins, Pam18 and Mdj2, can form the import motor. Both contain transmembrane and matrix domains, with Pam18 having an additional intermembrane space (IMS) domain. Evolutionary analyses revealed that the origin of the IMS domain of S. cerevisiae Pam18 coincides with a gene duplication event that generated the PAM18/MDJ2 gene pair. The duplication event and origin of the Pam18 IMS domain occurred at the relatively ancient divergence of the fungal subphylum Saccharomycotina. The timing of the duplication event also corresponds with a number of additional functional changes related to mitochondrial function and respiration. Physiological and genetic studies revealed that the IMS domain of Pam18 is required for efficient growth under anaerobic conditions, even though it is dispensable when oxygen is present. Thus, the gene duplication was beneficial for growth capacity under particular environmental conditions as well as diversification of the import motor components. |
| Databáze: | OpenAIRE |
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