Alterations in Aspergillus brasiliensis ( niger ) ATCC 9642 membranes associated to metabolism modifications during application of low-intensity electric current
| Autor: | Nancy Velasco-Alvarez, Mariano Gutiérrez-Rojas, Ignacio González |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Membrane permeability Biophysics 010501 environmental sciences Biology 01 natural sciences Cell membrane 03 medical and health sciences chemistry.chemical_compound Bioreactors Alkanes Electrochemistry medicine Biomass Physical and Theoretical Chemistry 0105 earth and related environmental sciences chemistry.chemical_classification Reactive oxygen species Cell Membrane Electric Conductivity General Medicine Metabolism Carbon Dioxide Spores Fungal Malondialdehyde Spore Oxygen 030104 developmental biology Mitochondrial respiratory chain medicine.anatomical_structure Membrane chemistry Biochemistry Aspergillus niger |
| Zdroj: | Bioelectrochemistry. 118:1-7 |
| ISSN: | 1567-5394 |
| Popis: | The effects of electric current on membranes associated with metabolism modifications in Aspergillus brasiliensis (niger) ATCC 9642 were studied. A 450-mL electrochemical cell with titanium ruthenium-oxide coated electrodes and packed with 15g of perlite, as inert support, was inoculated with A. brasiliensis spores and incubated in a solid inert-substrate culture (12 d; 30°C). Then, 4.5days after starting the culture, a current of 0.42mAcm-2 was applied for 24h. The application of low-intensity electric current increased the molecular oxygen consumption rate in the mitochondrial respiratory chain, resulting in high concentrations of reactive oxygen species, promoting high lipoperoxidation levels, according to measured malondialdehyde, and consequent alterations in membrane permeability explained the high n-hexadecane (HXD) degradation rates observed here (4.7-fold higher than cultures without current). Finally, cell differentiation and spore production were strongly stimulated. The study contributes to the understanding of the effect of current on the cell membrane and its association with HXD metabolism. |
| Databáze: | OpenAIRE |
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