Effects of TFAR19 gene on the in vivo biorheological properties and pathogenicity of mouse erythroleukemia cell line MEL
| Autor: | Weibo Ka, Dongqi He, Li Gu, Zhiyu Tang, Dagong Sun, Zongyao Wen |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Spleen Biology Basophil General Biochemistry Genetics and Molecular Biology Mice In vivo Cell Line Tumor Erythrocyte Deformability hemic and lymphatic diseases medicine Animals Erythroleukemia cell neoplasms Gene General Environmental Science Mice Inbred BALB C Pathogenicity Molecular biology Neoplasm Proteins carbohydrates (lipids) medicine.anatomical_structure Immunology Leukemia Erythroblastic Acute Bone marrow Apoptosis Regulatory Proteins Rheology General Agricultural and Biological Sciences |
| Zdroj: | Science in China Series C: Life Sciences. 50:111-119 |
| ISSN: | 1862-2798 1006-9305 |
| DOI: | 10.1007/s11427-007-0007-3 |
| Popis: | After injecting VP16, MEL cells and MEL-TF19 cells into the body of mice, with those injected with the same dose of saline as the control group, we observed the mice for their blood pictures, histological changes of the liver and spleen, and the hemorheological indexes within 4 weeks. The results indicated that after injecting MEL cells, the mice entered into a pathological status similar to erythroleukemia, which had the following exhibitions: the tissue structures of the liver and spleen were damaged, a mass of proerythroblasts, basophil erythroblasts and polychromatophilic erythroblasts could be observed on the smears of the bone marrow and spleen, and the deformability and orientation ability of erythrocytes were both depressed. The pathogenicity of MEL-TF19 cells carrying TFAR19 gene was obviously lower than that of MEL cells, and the MEL-TF19 cells even lost their faintish pathogenicity under the apoptosis-inducing effect of the chemotherapeutic reagent. The outcome from the animal experiments suggests that the TFAR19 gene suppresses the pathogenicity of MEL cells to the mice, and the effect may be better exerted with the synergy of the chemotherapeutic reagent. |
| Databáze: | OpenAIRE |
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