Neoplastic transformation by TERT in FGF-2-expanded human mesenchymal stem cells
| Autor: | Taijiro Sueda, Yusuke Sotomaru, Takeshi Sudo, Eiso Hiyama, Emi Yamaoka, Yoshiyuki Onitake, Ikuko Fukuba, Keiko Hiyama |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Cancer Research Telomerase Pathology medicine.medical_specialty Cellular differentiation Transplantation Heterologous Mice SCID Biology Mice Cancer stem cell medicine Animals Humans Telomerase reverse transcriptase Neoplastic transformation Cells Cultured Cell Proliferation Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells equipment and supplies Cell Transformation Neoplastic Oncology Cancer research Fibroblast Growth Factor 2 Stem cell Adult stem cell |
| Zdroj: | International journal of oncology. 39(1) |
| ISSN: | 1791-2423 |
| Popis: | The low percentage of human mesenchymal stem cells (hMSCs) in bone marrow necessitates their in vitro expansion prior to clinical use in regenerative medicine. We evaluated the effect of long-term culture of hMSCs on telomere length and transformation capacity by TERT transfection. hMSCs were isolated from the bone marrow aspirates of 24 donors and cultured with fibroblast growth factor-2 (FGF-2). Six cell lines with >500 population doubling levels were considered immortalized. TERT was transfected into two of the six lines for a comparison of telomere length, telomerase activity, differential capacity, colony formation capacity in soft agar and tumorigenicity in immunodeficient (NOD-SCID) mice. hMSC lines exhibited elongated telomeres without the activation of telomerase and retained multi-lineage differentiation potential upon chondrogenic or adipogenic differentiation, while non-immortalized hMSCs showed a marked reduction in telomere length in the differentiation process. Immortalized hMSCs showed anchorage-independence and formed tumors in NOD-SCID mice. Histologically, these tumors consisted of differentiated cells such as fat tissue and cartilage. Two TERT-transfected hMSC lines showed high rates of tumor formation in NOD-SCID mice. These tumors were histologically similar to teratocarcinoma without differentiated cells. These cells may provide a model for the origin of cancer stem cells from adult stem cells, and indicate the possibility that telomerase activation has a major role in the malignant transformation of human stem cells. These data suggest that adult hMSCs have a potential for neoplastic transformation and have implications for the use of hMSCs in tissue engineering and regenerative medicine. |
| Databáze: | OpenAIRE |
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