| Popis: |
Two new three-dimensional (3D) metal-organic frameworks, {[Ho(H2O)((CO2)3C24H15)]·7H2O}n (1) and {[Ho3(H2O)3((CO2)3C18H12N)3]·7H2O}n (2), have been prepared under solvothermal or hydrothermal conditions by using two C3-symmetrical organic ligands 4,4',4''-benzene-1,3,5-triyl-tri-benzoic acid (H3BTB) and 4,4′,4′′-nitrilotribenzoic acid (H3NTB). The in vitro anticancer activities of 1 and 2 were detected on human glioma cell line U251. The MTT assay and the IC50 values indicated that, even though both of these two compounds have anticancer ability, 1 shows a stronger inhibitory effect on cancer proliferation. The Annexin V-FITC/PI assay confirmed that 1 inhibits U251 cells proliferation via apoptosis. This apoptotic effect was mediated by increased ROS and activation of caspase-3 and caspase-8, which is determined by flow cytometer and western blot. We then verified that the anticancer effect of the two new synthesized compounds could be abolished by ROS inhibitor NAC. |
