Cell adhesion induces overexpression of chromodomain helicase/ATPase DNA binding protein 1-like gene (CHD1L) and contributes to cell adhesion-mediated drug resistance (CAM-DR) in multiple myeloma cells
| Autor: | Song He, Jingling Han, Jing Liu, Yunhua He, Yaxun Wu, Yangyu Ou, Fei Zhong, Xiaobing Miao, Yuchan Wang, Qiru Wang, Xiaohong Xu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Stromal cell Cell Bone Marrow Cells Biology CHD1L Chromodomain 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Cell Adhesion medicine Humans RNA Small Interfering Cell adhesion DNA Helicases Hematology Adhesion Molecular biology Up-Regulation DNA-Binding Proteins Fibronectin 030104 developmental biology medicine.anatomical_structure Oncology Drug Resistance Neoplasm Apoptosis 030220 oncology & carcinogenesis biology.protein Stromal Cells Apoptosis Regulatory Proteins Multiple Myeloma |
| Zdroj: | Leukemia Research. 47:54-62 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/j.leukres.2016.05.007 |
| Popis: | Previous studies have shown that chromodomain helicase/ATPase DNA binding protein 1-like gene (CHD1L) exerts its anti-apoptotic function in many solid cancers. However, its role in human multiple myeloma (MM) has not been thoroughly elucidated. In this study, we investigate the role of CHD1L in MM. Preliminarily, up-regulation and down-regulation assay verified that CHD1L exerts its anti-apoptotic role through the apoptotic pathway involving caspase-9-caspase-3 apoptotic pathway in MM cells. In addition, we determined that CHD1L expression is increased when MM cells were adhered to fibronectin (FN) or bone marrow stromal cell line HS-5 cells and cell adhesion assay indicated that CHD1L siRNA reversed the high cell adhesion rate. Consistent with the reduced adhesion rate, the cells translated to a compromised cell adhesion-mediated drug resistance (CAM-DR) phenotype in MM. In summary, we will propose strategies for developing a CHD1L inhibitor for potential treatment of MM. |
| Databáze: | OpenAIRE |
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