A discrepancy between the instantaneous and the overall collection efficiency of the Fenwal CS3000 for peripheral blood stem cell apheresis
| Autor: | Christopher A. Juttner, L. Bikto, Thorp D, Ann Canty, P. G. Dyson, David N. Haylock |
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| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Blood volume Hematopoietic stem cell transplantation Peripheral blood mononuclear cell Blood cell Blood Transfusion Autologous Neoplasms Antineoplastic Combined Chemotherapy Protocols Medicine Humans Thioguanine Bone Marrow Diseases Cyclophosphamide Colony-forming unit Myelosuppressive Chemotherapy Blood Cells business.industry Daunorubicin Cytarabine Hematopoietic Stem Cell Transplantation Blood Component Removal Hematology General Medicine Hematopoietic Stem Cells Surgery Blood Cell Count Leukemia Myeloid Acute medicine.anatomical_structure Evaluation Studies as Topic Plasmapheresis Nuclear medicine business |
| Zdroj: | Journal of clinical apheresis. 7(1) |
| ISSN: | 0733-2459 |
| Popis: | The collection efficiency (CE) of the Fenwall CS3000 continuous flow blood cell separator in the apheresis of peripheral blood stem cells during haemopoietic recovery following myelosuppressive chemotherapy was analysed. Ninety-three apheresis were performed in 19 patients using procedure 3 on the Fenwal CS3000. The overall CE was calculated from the pre-apheresis cell counts and the stated blood volume processed. Instantaneous CE was calculated from cell counts in the inlet and return lines. The overall mononuclear cell and granulocyte-macrophage colony forming unit CE were 64.0% and 55.8%, respectively, significantly lower than the instantaneous CEs of 94.5% and 95.4%, respectively (P = 0.0001, t test, for both comparisons). Three factors unrelated to machine performance contributed to the lower overall CE despite a high instantaneous CE: (1) A fall in the patient's mononuclear cell counts during apheresis leading to an overestimation of the cells available for collection, (2) dilution of blood by anti-coagulant, and (3) the operational dead space of the Fenwal CS3000. The overall CE corrected for these 3 factors approximated the instantaneous CE closely. Thus there is little room for further enhancement of machine performance because the Fenwal CS3000 is already operating with a very high instantaneous CE. To achieve major improvement in the yield of peripheral blood stem cell harvests, more effective mobilization protocols and better timing of apheresis are required. |
| Databáze: | OpenAIRE |
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