Classical Hodgkin lymphoma is characterized by high constitutive expression of activating transcription factor 3 (ATF3), which promotes viability of Hodgkin/Reed-Sternberg cells
| Autor: | Michael Hummel, Christian Hagemeier, Ralf C. Bargou, Stephan Mathas, Matthias Truss, Brigitte Wollert-Wulf, Korinna Jöhrens, Martin Janz, Harald Stein, Kurt Bommert, Bernd Dörken |
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| Rok vydání: | 2006 |
| Předmět: |
Cell Survival
Immunology Activating transcription factor Biology Biochemistry immune system diseases Cell Line Tumor hemic and lymphatic diseases medicine Humans RNA Small Interfering Reed-Sternberg Cells Transcription factor Cell Proliferation Oligonucleotide Array Sequence Analysis Regulation of gene expression ATF3 Activating Transcription Factor 3 Microarray analysis techniques Gene Expression Profiling Cell Biology Hematology medicine.disease Hodgkin Disease Gene Expression Regulation Neoplastic Gene expression profiling Reed–Sternberg cell Cell culture Cancer research |
| Zdroj: | Blood. 107:2536-2539 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Hodgkin/Reed-Sternberg (HRS) cells of classical Hodgkin lymphoma (cHL) display unique characteristics that discriminate cHL from other B-cell lymphomas and normal B cells. Therefore, comparative gene expression profiling of Hodgkin and non-Hodgkin B cells could lead to the identification of candidate genes that are critical for the pathogenesis of cHL. We performed microarray analysis of Hodgkin and non-Hodgkin cell lines and identified activating transcription factor 3 (ATF3), a member of the cyclic AMP response element binding protein (CREB)/ATF family, as a differentially expressed candidate gene. Extensive analysis of a large panel of cell lines, primary tumor samples, and normal tissues revealed that high expression of ATF3 is found in nearly all cases of cHL and is almost exclusively restricted to it. Selective knock-down of ATF3 by RNA interference suppressed proliferation and strongly reduced viability of Hodgkin cells. Thus, overexpression of ATF3 is a molecular hallmark of cHL that contributes to the malignant growth of HRS cells. |
| Databáze: | OpenAIRE |
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