Light-triggered switching of liposome surface charge directs delivery of membrane impermeable payloads in vivo
| Autor: | Michael S. Meijer, Stefan Vogel, Frederick Campbell, Gabriela Arias-Alpizar, Alexander Rabe, John van Noort, Alexander Kros, Redmar C. Vlieg, Sylvestre Bonnet, Li Kong, Panagiota Papadopoulou |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Science General Physics and Astronomy Nanoparticle 02 engineering and technology Nanomedicine/methods General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Cations/metabolism Drug Delivery Systems Phagocytosis In vivo Cations Drug Delivery Systems/methods Animals Surface charge Membranes/metabolism lcsh:Science Zebrafish Liposome Multidisciplinary Innate immune system Membranes Chemistry Macrophages Nanobiotechnology General Chemistry 021001 nanoscience & nanotechnology Coupling (electronics) 030104 developmental biology Membrane Nanomedicine Liposomes Drug delivery Biophysics Nanoparticles lcsh:Q Nanoparticles/chemistry 0210 nano-technology Liposomes/chemistry Intracellular |
| Zdroj: | Nature Communications Nature Communications, 11, 3638 Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020) Arias-Alpizar, G, Kong, L, Vlieg, R C, Rabe, A, Papadopoulou, P, Meijer, M S, Bonnet, S, Vogel, S, van Noort, J, Kros, A & Campbell, F 2020, ' Light-triggered switching of liposome surface charge directs delivery of membrane impermeable payloads in vivo ', Nature Communications, vol. 11, 3638 . https://doi.org/10.1038/s41467-020-17360-9 Nature Communications, 11(1). NATURE PUBLISHING GROUP |
| ISSN: | 2041-1723 |
| Popis: | Surface charge plays a fundamental role in determining the fate of a nanoparticle, and any encapsulated contents, in vivo. Herein, we describe, and visualise in real time, light-triggered switching of liposome surface charge, from neutral to cationic, in situ and in vivo (embryonic zebrafish). Prior to light activation, intravenously administered liposomes, composed of just two lipid reagents, freely circulate and successfully evade innate immune cells present in the fish. Upon in situ irradiation and surface charge switching, however, liposomes rapidly adsorb to, and are taken up by, endothelial cells and/or are phagocytosed by blood resident macrophages. Coupling complete external control of nanoparticle targeting together with the intracellular delivery of encapsulated (and membrane impermeable) cargos, these compositionally simple liposomes are proof that advanced nanoparticle function in vivo does not require increased design complexity but rather a thorough understanding of the fundamental nano-bio interactions involved. Surface charge plays an important role in determining nanoparticle fate in vivo. Here the authors report on the development of a light triggered charge switching liposome and demonstrate light triggered liposome targeting, uptake and payload delivery in a zebrafish model. |
| Databáze: | OpenAIRE |
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