Highly-branched poly(N-isopropyl acrylamide) functionalised with pendant Nile red and chain end vancomycin for the detection of Gram-positive bacteria
Autor: | Sheila MacNeil, Stephen Rimmer, Pavintorn Teratarantorn, Thomas Swift, Richard Hoskins, Maria G. Katsikogianni, Ian Douglas |
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Rok vydání: | 2019 |
Předmět: |
Staphylococcus aureus
Gram-positive bacteria 0206 medical engineering Acrylic Resins Biomedical Engineering Polymer architecture Peptide Stimuli responsive 02 engineering and technology Biochemistry Article Biomaterials chemistry.chemical_compound Vancomycin Polymer chemistry Diagnostic device Molecular Biology ComputingMethodologies_COMPUTERGRAPHICS chemistry.chemical_classification biology Nile red General Medicine Polymer 021001 nanoscience & nanotechnology biology.organism_classification 020601 biomedical engineering Fluorescence 3. Good health Solvatochromism chemistry Acrylamide Bacterial sensor Specificity 0210 nano-technology Bacteria Biotechnology |
Zdroj: | Acta Biomaterialia |
ISSN: | 1742-7061 |
Popis: | Graphical abstract This study shows how highly branched poly(N-isopropyl acrylamide) (HB-PNIPAM) with a chain pendant solvatochromic dye (Nile red) could provide a fluorescence signal, as end groups bind to bacteria and chain segments become desolvated, indicating the presence of bacteria. Vancomycin was attached to chain ends of HB-PNIPAM or as pendant groups on linear polymers each containing Nile red. Location of the dye was varied between placement in the core of the branched polymer coil or the outer domains. Both calorimetric and fluorescence data showed that branched polymers responded to binding of both the peptide target (D-Ala-D-Aa) and bacteria in a different manner than analogous linear polymers; binding and response was more extensive in the branched variant. The fluorescence data showed that only segments located in the outer domains of branched polymers responded to binding of Gram-positive bacteria with little response when linear analogous polymer or branched polymer with the dye in the inner core was exposed to Staphylococcus aureus. |
Databáze: | OpenAIRE |
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