Sequential interactions of fibroblast growth factor-2, brain-derived neurotrophic factor, neurotrophin-3, and their receptors define critical periods in the development of cochlear ganglion cells
| Autor: | W.Amin Hossain, Craig L. Brumwell, D.K. Morest |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
animal structures Time Factors Neurotrophin-3 Tropomyosin receptor kinase B Chick Embryo Tropomyosin receptor kinase A Tropomyosin receptor kinase C Developmental Neuroscience Neurotrophin 3 Neurotrophic factors Cell Movement Internal medicine medicine Neurites Low-affinity nerve growth factor receptor Animals Drug Interactions Receptor trkC RNA Messenger Antibodies Blocking Cells Cultured In Situ Hybridization Brain-derived neurotrophic factor Neurons biology Brain-Derived Neurotrophic Factor Stem Cells Immunohistochemistry Endocrinology nervous system Neurology embryonic structures biology.protein Fibroblast Growth Factor 2 Spiral Ganglion Neurotrophin |
| Zdroj: | Experimental neurology. 175(1) |
| ISSN: | 0014-4886 |
| Popis: | We studied the interactions of neurotrophin-3 (NT3) with brain-derived neurotrophic factor (BDNF), fibroblast growth factor-2 (FGF-2), and their effects on tyrosine kinase C (TrkC) expression during cochlear ganglion development. Otocysts were explanted from white leghorn chicken embryos at stages when the neuronal precursors normally start to migrate. Cultures were fed with various combinations of NT3, BDNF, and FGF-2. NT3 appeared to have a greater effect on neurite outgrowth than on migration and was enhanced by BDNF. The results from in situ hybridization and immunostaining for TrkC receptor revealed up-regulation of the mRNA and protein by combining NT-3 and BDNF. NT-3 combined with FGF-2 produced down-regulation of receptor. Neutralizing antibody to NT3 had an inhibitory effect on neuronal development, suggesting that endogenous NT3 is normally active during the period examined. The findings suggest an interactive role of NT3 in early neuronal development. The trophic synergism of NT3 and BDNF may result from up-regulation of TrkC. This hypothesis is consistent with immunostaining in the embryonic basilar papilla, which localized TrkC to the initial axonal invasion sites. While the growth factors each produce particular trophic effects, the interactions of these factors define a critical sequence of developmental events based on modulation of receptor expression. |
| Databáze: | OpenAIRE |
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