Potassium Assay in Blastomere of Two-Cell Mouse Embryo after Equilibration and washing from Cryoprotector
| Autor: | E. I. Smol’yaninova, D. V. Gol’dshtein, A. G. Pogorelov |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Blastomeres
Ethylene Glycol Potassium Cell Embryonic Development chemistry.chemical_element Mice Inbred Strains Biology General Biochemistry Genetics and Molecular Biology Cryopreservation Mice Cryoprotective Agents Electrolyte imbalance medicine Animals Electron probe microanalysis Water Embryo General Medicine Blastomere Embryo Mammalian medicine.disease Embryonic stem cell Molecular biology Cell biology Freeze Drying medicine.anatomical_structure chemistry Electron Probe Microanalysis |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 138:40-41 |
| ISSN: | 0007-4888 |
| DOI: | 10.1023/b:bebm.0000046933.90918.1d |
| Popis: | The need for effective methods of cryopreservation of early mammalian embryos necessitates the study of the mechanisms of blastomere adaptation to the equilibration procedure and subsequent washing from the cryoprotector. The osmotic effects during these procedures can cause electrolyte imbalance in embryonic cells. Intracellular potassium concentrations at the stage of two-blastomere mouse embryo were studied by electron probe microanalysis. |
| Databáze: | OpenAIRE |
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