Development and Characterization of Domain-Specific Monoclonal Antibodies Produced Against Human SLAMF9
| Autor: | Alexander M. Najakshin, O. V. Volkova, Nicolai A. Chikaev, Sergey V. Guselnikov, Sergey V. Kulemzin, Alexander V. Taranin, Evdokiya S. Reshetnikova, Ludmila V. Mechetina, Konstantin O Baranov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.drug_class
Immunology Immunoblotting Enzyme-Linked Immunosorbent Assay Receptors Cell Surface Monoclonal antibody Subclass Epitope Flow cytometry Mice Antigen Signaling Lymphocytic Activation Molecule Family Member 1 Antibody Specificity Antigens CD Signaling Lymphocytic Activation Molecule Family Cell Line Tumor medicine Escherichia coli Immunology and Allergy Animals Humans Receptor Mice Inbred BALB C biology medicine.diagnostic_test Antibodies Monoclonal Original Articles Flow Cytometry Molecular biology Immunohistochemistry Protein Structure Tertiary biology.protein Antibody Clone (B-cell biology) |
| Popis: | SLAMF9 is a member of the signaling lymphocyte-activating molecule (SLAM) immunoreceptor family. The SLAM family receptors are expressed in a broad range of immune cells and play an important role in immunity. To date, SLAMF9 is the least studied member of this family. Its ligand, signaling properties, and cells on whose surface it is expressed are unknown. We generated hybridoma clones 6E11 and 7G5 secreting monoclonal antibodies specific to human SLAMF9. BALB/c mice were immunized with Escherichia coli-expressed purified SLAMF9 protein; splenocytes from these mice were fused with mouse myeloma cell line NS-1. Based on isotyping of the MAbs, clone 6E11 was referred to the IgG1 subclass, while 7G5 to IgG2b. The specificity of these MAbs was assessed by ELISA, immunoblotting, immunohistochemistry, and flow cytometry. According to the results of epitope analysis, clone 6E11 reacts with the C2-like domain, whereas 7G5 is specific to the V-like domain of the SLAMF9 molecule. The generated MAbs were demonstrated to be applicable in various immunochemical analyses. They may be useful tools in studies clarifying the expression and function of human SLAMF9. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|