Calcified decellularized arterial scaffolds impact vascular smooth muscle cell transformation via downregulating α-SMA expression and upregulating OPN expression
| Autor: | Peng Wang, Xiaoxiao Tao, Huaping Xin, Liling You, Shuwu Wu, Zhimin Wang, Chenhua Xu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Vascular smooth muscle Decellularization biology Oncogene Chemistry Cell General Medicine Articles Molecular medicine Cell biology Extracellular matrix 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Immunology and Microbiology (miscellaneous) 030220 oncology & carcinogenesis medicine biology.protein Osteopontin Myofibroblast |
| Zdroj: | Experimental and therapeutic medicine. 18(1) |
| ISSN: | 1792-0981 |
| Popis: | The underlying mechanisms of arterial remodeling (AR) remain unclear. Studies have indicated that decellularized scaffolds stimulate the differentiation of fibroblasts into myofibroblasts and promote the accumulation of the extracellular matrix (ECM). In the present study, the impact of ECM changes following AR on vascular smooth muscle cell (VSMC) phenotypes was investigated. VSMCs were co-cultured with normal or calcified decellularized arterial scaffolds. The expression levels of α-smooth muscle actin (α-SMA) and osteopontin (OPN) were measured at 2, 5, 10, 15 and 21 days following the establishment of the co-culture systems. The expression of α-SMA in the normal co-culture group was significantly increased compared with that in the calcified arterial decellularized scaffold co-culture group (P |
| Databáze: | OpenAIRE |
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