FADD-deficient T cells exhibit a disaccord in regulation of the cell cycle machinery
| Autor: | Nisha H. Kabra, Chulho Kang, Jianke Zhang, Dragana Cado, Astar Winoto |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
CD4-Positive T-Lymphocytes
Fatty Acid Desaturases Receptor complex Time Factors T-Lymphocytes Blotting Western Receptors Antigen T-Cell Down-Regulation Muscle Proteins Apoptosis CD8-Positive T-Lymphocytes urologic and male genital diseases Lymphocyte Activation Biochemistry Mice Cyclin D2 Cyclins Animals FADD Receptor Cell Cycle Protein Molecular Biology biology Cell Death Kinase Arabidopsis Proteins Cell Cycle Microfilament Proteins Cell Biology Cell cycle Flow Cytometry Cell biology Blastocyst biology.protein biological phenomena cell phenomena and immunity Cell Division |
| Zdroj: | The Journal of biological chemistry. 276(32) |
| ISSN: | 0021-9258 |
| Popis: | FADD is an adapter protein that was originally isolated as a transducer of apoptotic signals for death domain-containing receptors. However, FADD-deficient mice are embryonic lethal and FADD(-/-) T cells developed from FADD(-/-) embryonic stem cells in the RAG-1(-/-) hosts lack the full potential to proliferate when stimulated through their T-cell receptor complex, suggesting that FADD protein might play a dualistic role in mediating not only cell death signaling but other non-apoptotic cellular pathways as well. Here we show that a substantial number of freshly isolated FADD(-/-) peripheral T cells are cycling but are defective in their co-stimulatory response when stimulated. Analysis of several cell cycle proteins shows normal down-regulation of p27 inhibitor but increased levels of p21, decreased levels of cyclin D2, and constitutive activation of several cyclin-dependent kinases in activated T cells. These data suggest that FADD is involved in the regulation of cell cycle machinery in T lymphocytes. |
| Databáze: | OpenAIRE |
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