Bystander Cell Death and Stress Response is Inhibited by the Radical Scavenger α1-Microglobulin in Irradiated Cell Cultures
| Autor: | Jan Paczesny, Sigurbjörg Rutardottir, Magnus G. Olsson, Bo Åkerström, E.J. Charlotta Nilsson, Jan Pallon |
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| Rok vydání: | 2010 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Programmed cell death Biophysics medicine.disease_cause Superoxide dismutase Cell Line Tumor Alpha-Globulins medicine Bystander effect Humans Radiology Nuclear Medicine and imaging Cell damage chemistry.chemical_classification Reactive oxygen species Radiation Cell Death biology Chemistry Dose-Response Relationship Radiation Bystander Effect Free Radical Scavengers Alpha Particles medicine.disease Molecular biology Up-Regulation Heme oxygenase Oxidative Stress Protein Transport Apoptosis biology.protein Tumor Suppressor Protein p53 Reactive Oxygen Species Biomarkers Oxidative stress |
| Zdroj: | Radiation Research. 174:590-600 |
| ISSN: | 1938-5404 0033-7587 |
| DOI: | 10.1667/rr2213.1 |
| Popis: | Alpha-particle irradiation of cells damages not only the irradiated cells but also nontargeted bystander cells. It has been proposed that the bystander effect is caused by oxidants and free radicals generated by the radiation. Recent studies have shown that α(1)-microglobulin protects against cell damage caused by oxidants and free radicals. Using a novel experimental system that allows irradiation of 0.02% of a human hepatoma monolayer, leaving 99.98% as bystander cells, we investigated the influence of oxidative stress and the cell-protective effects of α(1)-microglobulin during α-particle irradiation. The results showed an increase in cell death in both irradiated cells and bystander cells. A significant increase in apoptosis, oxidation markers and expression of the stress response genes heme oxygenase 1, superoxide dismutase, catalase, glutathione peroxidase 1, p21 and p53 were observed. Addition of α(1)-microglobulin reduced the amount of dead cells and inhibited apoptosis, formation of oxidation markers, and up-regulation of stress response genes. The results emphasize the role of oxidative stress in promoting bystander effects. Furthermore, the results suggest that α(1)-microglobulin protects nonirradiated cells by eliminating oxidants and free radicals generated by radiation and imply that α(1)-microglobulin can be used in radiation therapy of tumors to minimize damage to surrounding tissues. |
| Databáze: | OpenAIRE |
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