Local Zinc Chloride Release From a Calcium Sulfate Carrier Enhances Fracture Healing
| Autor: | J Patrick OʼConnor, Richard A Vincent, Kristen P Pacific, Jessica Cottrell, Jeremy Hreha, Joseph Benevenia, Sheldon S. Lin, Phillip H Lim, Joseph A. Ippolito, Suleiman Y Sudah, Maximilian F Muñoz, Ethan S. Krell, Nicholas J. Montemurro |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.medical_treatment Urology chemistry.chemical_element Bone healing Calcium Calcium Sulfate law.invention Intramedullary rod 03 medical and health sciences 0302 clinical medicine Chlorides law Tensile Strength medicine Animals Orthopedics and Sports Medicine Rats Wistar Saline Fracture Healing 030222 orthopedics Femur fracture Drug Carriers Dose-Response Relationship Drug business.industry Cartilage Femoral canal General Medicine Effective dose (pharmacology) Rats 030104 developmental biology medicine.anatomical_structure Treatment Outcome chemistry Zinc Compounds Delayed-Action Preparations Surgery business Femoral Fractures |
| Zdroj: | Journal of orthopaedic trauma. 31(3) |
| ISSN: | 1531-2291 |
| Popis: | Background This study examined the efficacy of calcium sulfate (CaSO4) as a carrier for intramedullary delivery of zinc chloride (ZnCl2) to treat fracture healing in a BB Wistar rat model. A non-carrier-mediated injection of 3.0 mg/kg of ZnCl2 has previously been shown to enhance fracture healing. Methods A heterogeneous mixture of ZnCl2 and CaSO4 was administered into the intramedullary femoral canal and a mid-diaphyseal femur fracture was created unilaterally. Early and late parameters of fracture healing were assessed using biomechanical testing, radiographic scoring, quantitative histomorphometry (for percentage of new cartilage and bone within the fracture callus), and long-term histologic evaluation. Results Fractures treated with 1.0 mg/kg of ZnCl2/CaSO4 demonstrated a significantly higher maximum torque to failure compared with both CaSO4 (P = 0.048) and saline (P = 0.005) controls at 4 weeks postfracture (396.4 versus 251.3 versus 178.7 N mm, respectively). Statistically significant increases in torsional rigidity, effective shear modulus, and effective shear stress were also found, as well as a 3.5 times increase in radiographic score (based on bone union). Histologic examination of the fracture callus indicated enhanced chondrogenesis at day 14 postfracture, with increased percent cartilage for the ZnCl2/CaSO4 group compared with saline (P = 0.0004) and CaSO4 (P = 0.0453) controls. Long-term radiographic and histologic evaluation revealed no abnormal bone formation or infection up to 12 weeks postoperatively. Conclusions The effective dose of ZnCl2 augmentation for the enhancement of fracture healing in rats was reduced 3-fold in this study compared with previous findings. Furthermore, CaSO4 acted synergistically with ZnCl2 to increase the mechanical strength and stability at the fracture site. |
| Databáze: | OpenAIRE |
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