Cell-cycle-dependent three-dimensional redistribution of nuclear proteins, P 120, pKi-67, and SC 35 splicing factor, in the presence of the topoisomerase I inhibitor camptothecin
| Autor: | Hélène Bobichon, Marianne Lorenzato, Emmanuel Elias, Nathalie Lalun, Pavel Chelidze, Laurent Blache, Dominique Ploton, Marie-Françoise O'Donohue |
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| Přispěvatelé: | Laboratoire de Biologie cellulaire, Histologie, Cytogénétique, UFR MEDECINE, Biomolécules : interactions moléculaires, cellulaires et cellules-matrice extracellulaire (BIMCCME), Université de Reims Champagne-Ardenne (URCA)-Centre Hospitalier Universitaire de Reims (CHU Reims)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biologie moléculaire eucaryote (LBME), Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA) |
| Rok vydání: | 2003 |
| Předmět: |
Cytoplasm
Nucleolus [SDV]Life Sciences [q-bio] [SDV.BC]Life Sciences [q-bio]/Cellular Biology Topoisomerase-I Inhibitor Culture Media Serum-Free 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Humans Enzyme Inhibitors Nuclear protein 030304 developmental biology Cell Nucleus 0303 health sciences Microscopy Confocal Nucleoplasm Serine-Arginine Splicing Factors biology Topoisomerase Cell Cycle G1 Phase Nuclear Proteins p120 GTPase Activating Protein Cell Biology Cell cycle Cell Compartmentation Cell biology Ki-67 Antigen DNA Topoisomerases Type I Ribonucleoproteins 030220 oncology & carcinogenesis biology.protein Camptothecin Topoisomerase I Inhibitors medicine.drug |
| Zdroj: | Experimental Cell Research Experimental Cell Research, Elsevier, 2003, 291 (1), pp.176-188. ⟨10.1016/s0014-4827(03)00377-x⟩ |
| ISSN: | 0014-4827 1090-2422 |
| Popis: | International audience; Topoisomerase I (Topo I) is mostly known for its role in DNA relaxation, which is required for duplication and transcription. Topo I acts as a protein kinase mainly directed to the mRNA splicing factor SC35. Camptothecin is one of the specific Topo I inhibitors and is effective on the two functions of the enzyme. In this study we demonstrated that treatment of KB cells with camptothecin for only 30 min induced the 3D reorganization and redistribution of three proteins involved in the nucleus machinery, P 120, pKi-67, and SC 35, and this occurred in a cell cycle-dependent manner. Our data were obtained from confocal microscopic studies after immunolabeling, 3D reconstruction, and measurement of the nuclear components volumes. In the presence of camptothecin, P 120, which occupied the nucleolar volume, lost its reticulation and pKi-67 was redistributed within the nucleoplasm and even into the cytoplasm. Finally, for SC 35 the fusion of its dots into bigger volumes was observed specifically during the G1 phase. Variations of volumes were also observed for the nucleolus and for the nucleus. These results pointed out that, depending on the cell cycle phase, Topo I functions were selective toward the three different proteins. |
| Databáze: | OpenAIRE |
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