Production of Human Monoclonal Antibodies in Dialysis Tubing
| Autor: | Eva Sjögren-Jansson, Stig Jeansson, Carl A.K. Borrebaeck, Mats Ohlin |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Hybridomas
Anticorps monoclonal biology medicine.drug_class Immunology Antibodies Monoclonal Elisa assay Laboratory scale Monoclonal antibody Molecular biology Culture Media Dialysis tubing Mice Tissue culture Cell culture Immunologic Techniques Genetics medicine biology.protein Animals Humans Antibody Dialysis Cell Division |
| Zdroj: | Hybridoma. 10:411-419 |
| ISSN: | 0272-457X |
| Popis: | Human x mouse hybridoma cells were grown in dialysis tubing (DT) to obtain large quantities of human monoclonal antibodies (MAb). Hybridoma cells were grown inside the DT, which was placed in a tissue culture flask containing medium. The medium inside the DT was supplemented with different additives which may be selected depending on the intended use of the MAb. About 10-50 times higher concentrations of immunoglobulin (Ig) were obtained after cultivation in DT compared to conventional tissue culture (CTC) for 2 days. The purity of the MAb was high which facilitated further antibody purification. Production of human MAb in DT proved to be excellent for evaluation studies in laboratory scale. It does not require expensive equipment and several hybridomas can be grown simultaneously. |
| Databáze: | OpenAIRE |
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