Human Gingival Crevicular Fluid Contains MRP8 (S100A8) and MRP14 (S100A9), Two Calcium-binding Proteins of the S100 Family
| Autor: | E. Andersen, Denis F. Hochstrasser, William F. Pralong, Marc R. Wilkins, T. Kojima, G. Cimasoni, Jean-Charles Sanchez |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
0301 basic medicine Saliva Silver Blotting Western Peptide Mapping Mass Spectrometry 03 medical and health sciences 0302 clinical medicine Peptide mass fingerprinting Western blot Sequence Analysis Protein medicine Calgranulin B Humans Calgranulin A Electrophoresis Gel Two-Dimensional Isoelectric Point Gingival sulcus Coloring Agents Periodontitis General Dentistry Polyacrylamide gel electrophoresis Aged Mouth medicine.diagnostic_test Chemistry Lasers Calcium-Binding Proteins S100 Proteins Infant Newborn Infant Gingival Crevicular Fluid 030206 dentistry Middle Aged medicine.disease Antigens Differentiation Molecular biology Molecular Weight Blot 030104 developmental biology Isoelectric point Mouth Edentulous Biomarkers |
| Zdroj: | Journal of Dental Research. 79:740-747 |
| ISSN: | 1544-0591 0022-0345 |
| Popis: | Human gingival crevicular fluid contains unidentified proteins which might play a role as markers in periodontal diseases. Therefore, low-molecular-weight proteins found in human gingival crevicular fluid (GCF), but absent from serum, were identified in the present study by means of two-dimensional electrophoresis (2-D PAGE) analysis. GCF, serum, and whole saliva were collected from periodontitis and healthy subjects, as well as from edentulous and newborn subjects. Protein samples were separated by two-dimensional polyacrylamide gel electrophoresis, stained with silver, and compared with reference protein maps in the SWISS-2D PAGE database. In GCF and saliva from periodontitis patients and healthy subjects, four dominant low-molecular-mass (from 8 to 14 kDa) acidic spots were observed. They were not found in serum and were less visible in saliva from edentulous and newborn subjects. From N-terminal amino acid sequencing, the two 2-D protein spots of 8 kDa and isoelectric points between 6.5 and 7.0 were both identified as protein MRP8 (S100A8), a member of the S100 family of calcium-binding proteins. Using peptide mass fingerprinting and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), we identified the other two protein spots, with mass of 14 kDa and isoelectric points between 5.5 and 6.0, as protein MRP14 (S100A9), also belonging to the S100 family. The presence of MRP8 and MRP14 in GCF was confirmed by Western blot, with monoclonal antibodies. The two polypeptides, MRP8 and MRP14, identified in GCF represent the major difference between the 2-D PAGE patterns of serum and GCF, and we hypothesize that they may play an important role in the gingival sulcus and could represent possible markers for periodontal diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|