Isolation of alligator gar (Lepisosteus spatula) glucagon, oxyntomodulin, and glucagon-like peptide: amino acid sequences of oxyntomodulin and glucagon-like peptide
| Autor: | Joe R. Kimmel, H.G. Pollock, Kurt E. Ebner, J.B. Rouse, Allen B. Rawitch, J.W. Hamilton, Valentine A. Lance |
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| Rok vydání: | 1988 |
| Předmět: |
Male
endocrine system Stereochemistry Molecular Sequence Data Glucagon-Like Peptides Radioimmunoassay Peptide Lepisosteus Peptide hormone Alligator gar Glucagon Gastrointestinal Hormones chemistry.chemical_compound Islets of Langerhans Endocrinology Animals Amino Acid Sequence Amino acid residue Amino Acids Chromatography High Pressure Liquid chemistry.chemical_classification biology digestive oral and skin physiology Fishes biology.organism_classification Amino acid Oxyntomodulin chemistry Biochemistry Chromatography Gel Animal Science and Zoology Female Peptides hormones hormone substitutes and hormone antagonists |
| Zdroj: | General and comparative endocrinology. 69(1) |
| ISSN: | 0016-6480 |
| Popis: | Oxyntomodulin, glucagon, and a glucagon-like peptide (GLP) have been isolated from the endocrine pancreas of the alligator gar (Lepisosteus spatula), a ganoid fish. The three peptides were isolated by gel filtration and HPLC and were identified by size, composition, and glucagon-like immunoreactivity. The amino acid sequences of the oxyntomodulin and GLP were determined. The oxyntomodulin contains 36 amino acid residues and its sequence is H S Q G T F T N D Y S K Y L D T R R A Q D F V Q W L M S T K R S T K R S G G I T. The composition of the glucagon is identical to the N-terminal 29 residues of the gar oxyntomodulin. The single form of GLP found contains 34 amino acid residues in the following sequence: H A D G T Y Y S D V S S Y L Q D Q A A K K F V T W L K Q B Q D R R E. These findings sugest that all three peptides are derived from a common precursor. |
| Databáze: | OpenAIRE |
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