A new method for phenotyping red blood cells using microplates
| Autor: | F. Llopis, F. Carbonell-Uberos, M.C. Montero, S. Bonanad, M.D. Planelles, I. Plasencia, C. Riol, T. Planells, C. Carrillo, A. Miguel |
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| Rok vydání: | 1999 |
| Předmět: |
Microbiological Techniques
Erythrocytes Reproducibility of Results Blood Donors hemic and immune systems Hematology General Medicine Sensitivity and Specificity Antibodies Immunophenotyping Antigen-Antibody Reactions Coombs Test Immunoglobulin G Antigens Surface Blood Group Antigens Humans circulatory and respiratory physiology |
| Zdroj: | VOX SANGUINIS r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname |
| ISSN: | 0042-9007 |
| Popis: | The supply of phenotyped red blood cells (RBC) for patients with several RBC antibodies presents a difficult task to hospital blood banks and regional blood centers. The aim of this study was to establish a low-cost typing system to allow extensive phenotyping of regular blood donors for clinically significant RBC antigens.We developed a new buffer that greatly intensifies the antigen-antibody reaction and thus reduces the quantity of serum needed for phenotyping. The procedure was carried out on microplates.A total of 20,435 regular blood donors have been typed to date. For 752 units required for transfusion, 3,584 phenotyping tests were performed, validating the results by tube or gel typing methods; agreement was achieved in all cases.This technique seems adequate for phenotyping a large number of RBC units at very low cost, thus facilitating the availability of phenotyped blood. |
| Databáze: | OpenAIRE |
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