Gi protein mediates adenylate cyclase inhibition by neurohypophyseal hormones in fish gill
| Autor: | Marielle Guibbolini, Brahim Lahlou |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Gills
endocrine system medicine.medical_specialty animal structures GTP' Physiology G protein Trout Adenylate kinase Biology Pertussis toxin Oxytocin Biochemistry Cyclase Cellular and Molecular Neuroscience Endocrinology Vasotocin Cell surface receptor GTP-Binding Proteins Internal medicine medicine Animals Virulence Factors Bordetella Guanine Nucleotides Pertussis Toxin Adenylyl Cyclase Inhibitors Adenylate Cyclase Toxin Cyclase activity Fish gill |
| Zdroj: | Peptides. 13(5) |
| ISSN: | 0196-9781 |
| Popis: | Adenylate cyclase activity was measured on membrane fractions from the gill epithelium of rainbow trout Salmo gairdneri. Basal and glucagon-stimulated activities responded negatively to homologous neurohypophyseal peptides (arginine-vasotocin and isotocin). This inhibitory effect was totally abolished in the presence of pertussis toxin (IAP). The guanine nucleotide dependence of the enzyme was further explored by using GTP, GDP, and their stable analogs Gpp(NH)p, GTPγS, and GDPβS. The results suggest that neurohypophyseal peptides at low concentrations inhibit the adenylate cyclase system directly by way of a Gi-protein, thus implying the intervention of a new type of membrane receptor for these hormones in fish gills. |
| Databáze: | OpenAIRE |
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