Rhodanese from Thiobacillus A2: determination of activity by proton nuclear magnetic resonance spectroscopy
| Autor: | O.W. Howarth, Marvin Silver, D.P. Kelly |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Dihydrolipoamide
Magnetic Resonance Spectroscopy ved/biology.organism_classification_rank.species Thiosulfates Rhodanese Microbiology Thiobacillus chemistry.chemical_compound Nuclear magnetic resonance medicine Moiety Spectroscopy Cell-Free System Thioctic Acid Chemistry ved/biology Nuclear magnetic resonance spectroscopy equipment and supplies Amides Thiosulfate Sulfurtransferase Sulfurtransferases Lipoamide Proton NMR medicine.drug Nuclear chemistry |
| Zdroj: | Journal of general microbiology. 97(2) |
| ISSN: | 0022-1287 |
| Popis: | Rhodanese from Thiobacillus A2 was shown by proton nuclear magnetic resonance (NMR) spectroscopy to use dihydrolipoate or dihydrolipoamide as acceptor of the sulphane moiety of thiosulphate with the formation of alpha-lipoate or lipoamide respectively. Correlation is shown between assays of the enzyme activity by NMR spectroscopy and by ultraviolet spectrophotometry. |
| Databáze: | OpenAIRE |
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