Fractionation and structural elucidation of the active components of aurintricarboxylic acid, a potent inhibitor of protein nucleic acid interactions
| Autor: | R.Gilberto González, Thomas Schleich, Barry J. Blackburn |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
chemistry.chemical_classification
Poly U Triphenylmethane dye Magnetic Resonance Spectroscopy Cyclohexanecarboxylic Acids Spectrum Analysis Aurintricarboxylic Acid Active components Ultrafiltration Polymer Fractionation DNA Biochemistry Genetics and Molecular Biology (miscellaneous) Molecular Weight chemistry.chemical_compound Structure-Activity Relationship chemistry Biochemistry Aurintricarboxylic acid Nucleic acid Escherichia coli Potency Polylysine Ribosomes |
| Zdroj: | Biochimica et biophysica acta. 562(3) |
| ISSN: | 0006-3002 |
| Popis: | Commercially available, as well as synthetically prepared, samples of aurintricarboxylic acid (a widely employed potent inhibitor of protein nucleic acid interactions) consist mostly of a heterogeneous collection of polymers, as shown by fractionation schemes utilizing both dialysis and ultrafiltration, and by molecular weight measurements. 13C-NMR studies suggest that the polymeric material is of the phenol-formaldehyde type; inhibitory assays that depend on the formation of a protein-nucleic acid complex revealed that potency varied directly with the molecular weight of the polymer. Fractions of molecular weight 400 were essentially inactive. |
| Databáze: | OpenAIRE |
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