Anti-D monoclonal antibodies from 23 human and rodent cell lines display diverse IgG Fc-glycosylation profiles that determine their clinical efficacy
| Autor: | Jodie L. Abrahams, Radka Saldova, Rick Kapur, Agnes L. Hipgrave Ederveen, Natalia I. Olovnikova, Kathryn L. Armour, Manfred Wuhrer, Gestur Vidarsson, Belinda Kumpel, Carolien A. M. Koeleman, Pauline M. Rudd |
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| Přispěvatelé: | Ederveen, Agnes Hipgrave [0000-0003-1689-0442], Vidarsson, Gestur [0000-0001-5621-003X], Kapur, Rick [0000-0002-1608-876X], Wuhrer, Manfred [0000-0002-0814-4995], Apollo - University of Cambridge Repository, AII - Inflammatory diseases, AII - Cancer immunology, Landsteiner Laboratory |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Proteomics Glycosylation medicine.drug_class Rho(D) Immune Globulin Glycobiology lcsh:Medicine Drug development 030204 cardiovascular system & hematology Monoclonal antibody Article Cell Line Erythroblastosis Fetal 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Cricetulus In vivo medicine Animals Humans lcsh:Science Fucosylation Fucose Antibody-dependent cell-mediated cytotoxicity Multidisciplinary Hybridomas biology Chinese hamster ovary cell lcsh:R Antibodies Monoclonal Galactose Molecular biology N-Acetylneuraminic Acid 3. Good health Rats 030104 developmental biology Treatment Outcome chemistry Cell culture Immunoglobulin G biology.protein lcsh:Q Antibody Immunosuppression Haematological diseases |
| Zdroj: | Scientific Reports Scientific Reports, 10(1). NATURE PUBLISHING GROUP Scientific reports, 10(1):1464. Nature Publishing Group Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
| DOI: | 10.1038/s41598-019-57393-9 |
| Popis: | Anti-D immunoglobulin (Anti-D Ig) prophylaxis prevents haemolytic disease of the fetus and newborn. Monoclonal IgG anti-Ds (mAb-Ds) would enable unlimited supplies but have differed in efficacy in FcγRIIIa-mediated ADCC assays and clinical trials. Structural variations of the oligosaccharide chains of mAb-Ds are hypothesised to be responsible. Quantitative data on 12 Fc-glycosylation features of 23 mAb-Ds (12 clones, 5 produced from multiple cell lines) and one blood donor-derived anti-D Ig were obtained by HPLC and mass spectrometry using 3 methods. Glycosylation of mAb-Ds from human B-lymphoblastoid cell lines (B) was similar to anti-D Ig although fucosylation varied, affecting ADCC activity. In vivo, two B mAb-Ds with 77–81% fucosylation cleared red cells and prevented D-immunisation but less effectively than anti-D Ig. High fucosylation (>89%) of mouse-human heterohybridoma (HH) and Chinese hamster ovary (CHO) mAb-Ds blocked ADCC and clearance. Rat YB2/0 mAb-Ds with 60%) together with lower fucosylation ( |
| Databáze: | OpenAIRE |
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