LncRNA HCG11/miR‐26b‐5p/QKI5 feedback loop reversed high glucose‐induced proliferation and angiogenesis inhibition of HUVECs
| Autor: | Li Yihua, Li Ya, Kai Sun, Yuan Xuchun, Zhaolong Xu, Liu Xiaolin, Bin Lu, Cai Zhenyu, Shurong Liu, Jiao Du, Xiuhai Guo, Ruijuan Han |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Angiogenesis Immunoprecipitation Neovascularization Physiologic medicine.disease_cause Neovascularization angiogenesis 03 medical and health sciences 0302 clinical medicine Genes Reporter Cell Line Tumor Human Umbilical Vein Endothelial Cells medicine Humans miR‐26b‐5p Cells Cultured Aged Tube formation QKI‐5 lncRNA HCG11 Chemistry Competing endogenous RNA Cell growth RNA-Binding Proteins RNA Original Articles Cell Biology Middle Aged Atherosclerosis Vulnerable plaque Cell biology MicroRNAs Glucose 030104 developmental biology Gene Expression Regulation 030220 oncology & carcinogenesis Molecular Medicine Original Article Female RNA Interference RNA Long Noncoding medicine.symptom Biomarkers |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| Popis: | Acute coronary syndrome caused by the rupture of atherosclerotic plaques is one of the primary causes of cerebrovascular and cardiovascular events. Neovascularization within the plaque is closely associated with its stability. Long non‐coding RNA (lncRNA) serves a crucial role in regulating vascular endothelial cells (VECs) proliferation and angiogenesis. In this study, we identified lncRNA HCG11, which is highly expressed in patients with vulnerable plaque compared with stable plaque. Then, functional experiments showed that HCG11 reversed high glucose‐induced vascular endothelial injury through increased cell proliferation and tube formation. Meanwhile, vascular‐related RNA‐binding protein QKI5 was greatly activated. Luciferase reporter assays and RNA‐binding protein immunoprecipitation (RIP) assays verified interaction between them. Interestingly, HCG11 can also positively regulated by QKI5. Bioinformatics analysis and luciferase reporter assays showed HCG11 can worked as a competing endogenous RNA by sponging miR‐26b‐5p, and QKI5 was speculated as the target of miR‐26b‐5p. Taken together, our findings revered that the feedback loop of lncRNA HCG11/miR‐26b‐5p/QKI‐5 played a vital role in the physiological function of HUVECs, and this also provide a potential target for therapeutic strategies of As. |
| Databáze: | OpenAIRE |
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