Effects of RNAi-mediated MUC4 gene silencing on the proliferation and migration of human pancreatic carcinoma BxPC-3 cells
| Autor: | Yu Pu, Xiao-Ming Zhang, Nan-lin Zeng, Changqiang Wu, Chengyi Shen, Tian-wu Chen, Gang Liu, Yang Zhang, Yong Li, Juan Juan Zhang, Jiang Zhu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Mice Nude Biology Small hairpin RNA 03 medical and health sciences 0302 clinical medicine Pancreatic tumor RNA interference Cell Movement Cell Line Tumor medicine Gene silencing Animals Humans RNA Small Interfering Cell Proliferation Gene knockdown Oncogene Mucin-4 General Medicine Transfection medicine.disease Molecular biology Tumor Burden Gene Expression Regulation Neoplastic Pancreatic Neoplasms 030104 developmental biology Oncology Cell culture 030220 oncology & carcinogenesis Cancer research RNA Interference sense organs Neoplasm Transplantation |
| Zdroj: | Oncology reports. 36(6) |
| ISSN: | 1791-2431 |
| Popis: | It was previously demonstrated that mucin 4 (MUC4) is not expressed in normal pancreatic tissues or in chronic pancreatitis tissue but is highly expressed in pancreatic cancer (PC) tissue. Effective MUC4 gene knockdown in PC may contribute to the elucidation of pancreatic tumor development and metastasis, and may be valuable in new therapeutic approaches. Thus to confirm this, in the present study, the BxPC-3 cell line was transfected with eight pairs of shRNA lentiviral vectors for MUC4. The qPCR results showed that expression of MUC4 mRNA in the BxPC-3 cells was significantly decreased at 96 h after transfection. One of these shRNA lentiviral vectors (shRNA‑A141) had showed the strongest suppressive effect on MUC4 mRNA expression and was used for MUC4 knockdown in BxPC-3 cells. After stable transfection, BxPC-3 cells showed a significantly lower expression of MUC4 mRNA and MUC4 protein, and were suppressed on cell growth and migration. In vivo, lower tumor growth rates and tumor volume were observed in the tumors derived from the MUC4-knockdown cells, whereas the transplanted tumors derived from the control group cells, grew rapidly. Thus, inhibition of MUC4 expression may be an effective means for mitigating metastasis and invasion of PC. |
| Databáze: | OpenAIRE |
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