Evaluating the Efficiency of DNA Metabarcoding to Analyze the Diet of Hippocampus Guttulatus (Teleostea: Syngnathidae)
| Autor: | Graziano Pesole, Cataldo Pierri, Bruno Fosso, Marinella Marzano, Frine Cardone, Bachir Balech, Monica Santamaria, Tamara Lazic, M. Deflorio, Carmela Gissi, Michele Gristina, Giuseppe Corriero, Francesco Nonnis Marzano |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Amphipoda
Science Paleontology Zoology Biology biology.organism_classification General Biochemistry Genetics and Molecular Biology Article Predation DNA metabarcoding Syngnathidae Isopoda seahorses Space and Planetary Science Seahorse Flagship species Conservation status Hippocampus guttulatus diet Ecology Evolution Behavior and Systematics |
| Zdroj: | Life Volume 11 Issue 10 Life (Basel) 11 (2021). doi:10.3390/life11100998 info:cnr-pdr/source/autori:Lazic, Tamara; Pierri, Cataldo; Corriero, Giuseppe; Balech, Bachir; Cardone, Frine; Deflorio, Michele; Fosso, Bruno; Gissi, Carmela; Marzano, Marinella; Nonnis Marzano, Francesco; Pesole, Graziano; Santamaria, Monica; Gristina, Michele/titolo:Evaluating the Efficiency of DNA Metabarcoding to Analyze the Diet of Hippocampus guttulatus (Teleostea: Syngnathidae)/doi:10.3390%2Flife11100998/rivista:Life (Basel)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:11 Life, Vol 11, Iss 998, p 998 (2021) |
| ISSN: | 2075-1729 |
| DOI: | 10.3390/life11100998 |
| Popis: | Seahorses are considered a flagship species for conservation efforts and due to their conservation status, improving knowledge on their dietary composition while applying a non-invasive approach, could be useful. Using Hippocampus guttulatus as a case study, the present study represents pioneering research into investigating the diet of seahorses by NGS-based DNA metabarcoding of fecal samples. The study developed and tested the protocol for fecal DNA metabarcoding during the feeding trials where captive seahorses were fed on a diet of known composition the process was subsequently applied on fecal samples collected from wild individuals. The analysis of samples collected during the feeding trials indicated the reliability of the applied molecular approach by allowing the characterization of the effectively ingested prey. In the field study, among detected prey species, results revealed that the majority of the seahorse samples contained taxa such as Amphipoda, Decapoda, Isopoda, and Calanoida, while less common prey taxa were Gastropoda and Polyplacophora. As only a small amount of starting fecal material is needed and the sampling procedure is neither invasive nor lethal. The present study indicates DNA metabarcoding as useful for investigating seahorse diet and could help define management and conservation actions. |
| Databáze: | OpenAIRE |
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