Effect of fludarabine on leukocyte functions
| Autor: | Ediz Demirpençe, Hande Canpinar, A. Lale Doğan, Ayça Doğan, Övünç Düzgünçınar |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Hypochlorous acid
Cell Survival Neutrophils Neutrophile Chronic lymphocytic leukemia Cell Culture Techniques Antineoplastic Agents HL-60 Cells Pharmacology Granulocyte Cell Degranulation chemistry.chemical_compound Drug Discovery medicine Humans Pharmacology (medical) Peroxidase biology Dose-Response Relationship Drug Chemistry Degranulation General Medicine medicine.disease Flow Cytometry Fludarabine Hypochlorous Acid Enzyme Activation Infectious Diseases medicine.anatomical_structure Oncology Apoptosis Myeloperoxidase Immunology biology.protein Vidarabine medicine.drug |
| Zdroj: | Chemotherapy. 50(6) |
| ISSN: | 0009-3157 |
| Popis: | Background: Fludarabine induces leukemic cell apoptosis and is highly efficient in chronic lymphocytic leukemia. However, fludarabine therapy causes severe leukopenia. Leukocyte myeloperoxidase (MPO) catalyzes the formation of HOCl, and this is the main microbicidal function in phagocytes. The aim of our study was to evaluate the effect of fludarabine on leukocytes, i.e. their degranulation capacity, MPO activity and HOCl production. Methods: Peripheral blood leukocytes were incubated for 48 h with fludarabine. Degranulation was measured using a flow-cytometric method. MPO activity and HOCl production were measured spectrophotometrically. Results: The degranulation capacity of fludarabine-treated leukocytes was significantly elevated compared to untreated controls. MPO activity and HOCl production were also increased in parallel. A possible direct activating effect of fludarabine was tested on the MPO activity of HL60 cells. Fludarabine did not affect MPO activity at concentrations ranging from 10 µM to 2 mM. Conclusion: Fludarabine had no inhibitory effect on the microbial killing of leukocytes. |
| Databáze: | OpenAIRE |
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