Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2
| Autor: | Volker M. Stucke, Silvia Martin-Lluesma, Erich A. Nigg |
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| Rok vydání: | 2002 |
| Předmět: |
RNA
Untranslated Cell cycle checkpoint Mad2 Mad1 Chromosomal Proteins Non-Histone Mitosis Cell Cycle Proteins Spindle Apparatus Protein Serine-Threonine Kinases Biology Microtubules Two-Hybrid System Techniques Chromosomes Human Humans Gene Silencing RNA Small Interfering Kinetochores Multidisciplinary Kinetochore Calcium-Binding Proteins Nuclear Proteins Protein-Tyrosine Kinases G2-M DNA damage checkpoint Cell biology Spindle apparatus Repressor Proteins Cytoskeletal Proteins Spindle checkpoint Phenotype Mitotic exit Mad2 Proteins biological phenomena cell phenomena and immunity Microtubule-Associated Proteins Protein Kinases HeLa Cells Signal Transduction |
| Zdroj: | Science. 297:2267-2270 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | The spindle checkpoint delays sister chromatid separation until all chromosomes have undergone bipolar spindle attachment. Checkpoint failure may result in chromosome mis-segregation and may contribute to tumorigenesis. We showed that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. Depletion of Hec1 impaired chromosome congression and caused persistent activation of the spindle checkpoint, indicating that high steady-state levels of Mad1/Mad2 complexes at kinetochores were not essential for checkpoint signaling. Simultaneous depletion of Hec1 and Mad2 caused catastrophic mitotic exit, making Hec1 an attractive target for the selective elimination of spindle checkpoint–deficient cells. |
| Databáze: | OpenAIRE |
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