Neuronal plasticity after spinal cord injury: identification of a gene cluster driving neurite outgrowth
| Autor: | Andrea De Biase, Barbara S. Bregman, Simone Di Giovanni, Susan M. Knoblach, Alan I. Faden, Tom Finn, Alexander G. Yakovlev, Melissa M. Thouin, Eric P. Hoffman, Jonathan S. Duke-Cohan |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Time Factors Transcription Genetic Neurite Biology Biochemistry Rats Sprague-Dawley Myelin GAP-43 Protein Dorsal root ganglion Ganglia Spinal Gene cluster Neurites Genetics medicine Animals RNA Messenger Molecular Biology Spinal cord injury Myelin Sheath Spinal Cord Injuries Oligonucleotide Array Sequence Analysis Neurons Neuronal Plasticity Gene Expression Profiling Membrane Proteins Anatomy Spinal cord medicine.disease Axons Oligodendrocyte Nerve Regeneration Rats Cell biology Myelin-Associated Glycoprotein medicine.anatomical_structure Gene Expression Regulation Multigene Family GDF7 Myelin-Oligodendrocyte Glycoprotein Microtubule-Associated Proteins Myelin Proteins Biotechnology |
| Zdroj: | The FASEB Journal. 19:153-154 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.04-2694fje |
| Popis: | Functional recovery after spinal cord injury (SCI) may result in part from axon outgrowth and related plasticity through coordinated changes at the molecular level. We employed microarray analysis to identify a subset of genes the expression patterns of which were temporally coregulated and correlated to functional recovery after SCI. Steady-state mRNA levels of this synchronously regulated gene cluster were depressed in both ventral and dorsal horn neurons within 24 h after injury, followed by strong re-induction during the following 2 wk, which paralleled functional recovery. The identified cluster includes neuritin, attractin, microtubule-associated protein 1a, and myelin oligodendrocyte protein genes. Transcriptional and protein regulation of this novel gene cluster was also evaluated in spinal cord tissue and in single neurons and was shown to play a role in axonal plasticity. Finally, in vitro transfection experiments in primary dorsal root ganglion cells showed that cluster members act synergistically to drive neurite outgrowth. |
| Databáze: | OpenAIRE |
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