Comparison of the sensitivity of the protein A plaque assay and the immunofluorescence assay for detection of immunoglobulin producing cells
| Autor: | A. Van Oudenaren, J J Haaijman, Robbert Benner |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Aging
Immunology Fluorescent Antibody Technique Hemolytic Plaque Technique Biology Immunofluorescence Mice Antigen medicine Animals Immunology and Allergy Bioassay Antibody-Producing Cells Staphylococcal Protein A Virus quantification Mice Inbred BALB C medicine.diagnostic_test Cell Differentiation Molecular biology Lymphatic system Cytoplasm biology.protein Female Antibody Protein A Spleen |
| Zdroj: | Journal of Immunological Methods. 56:117-124 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(83)90054-6 |
| Popis: | This paper reports a comparative study on the sensitivity of the protein A plaque assay and the cytoplasmic immunofluorescence assay for detection of immunoglobulin (Ig) producing cells in cell suspensions of murine lymphoid organs. The protein A plaque assay was found to detect as many or several times more Ig producing cells than the immunofluorescence assay, depending on the age and antigenic load of the mice, and upon the Ig class and organ studied. |
| Databáze: | OpenAIRE |
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