The primary mode of binding of cisplatin to a B-DNA dodecamer: C-G-C-G-A-A-T-T-C-G-C-G
| Autor: | R.M. Wing, H.R. Drew, Richard E. Dickerson, P.E. Pjura |
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| Rok vydání: | 1984 |
| Předmět: |
Models
Molecular Chemical Phenomena Stereochemistry Guanine Oligonucleotides Biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound X-Ray Diffraction medicine Glycosyl Binding site Transversion Molecular Biology Cisplatin General Immunology and Microbiology Base Sequence General Neuroscience DNA Chemistry Dodecameric protein chemistry Biochemistry Oligodeoxyribonucleotides Depurination Nucleic Acid Conformation medicine.drug Research Article |
| Zdroj: | The EMBO journal. 3(5) |
| ISSN: | 0261-4189 |
| Popis: | When cisplatin [cis- diamminodichloroplatinum (II)] is diffused into pre-grown crystals of the B-DNA double-helical dodecamer C-G-C-G-A-A-T-T-C-G-C-G, it binds preferentially to the N7 positions of guanines, with what probably is an aquo bridge between Pt and the adjacent O6 atom of the same guanine. The entire guanine ring moves slightly toward the platinum site, into the major groove. Only three of the eight potential cisplatin binding sites on guanines actually are occupied, and this differential reactivity can be explained in terms of the relative freedom of motion of guanines toward the major groove. This shift of guanines upon ligation may weaken the glycosyl bond and assist in the depurination that leads to mismatch SOS repair and G.C. to T.A. transversion. |
| Databáze: | OpenAIRE |
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