In situ, chemical and macromolecular study of the composition of Arabidopsis thaliana seed coat mucilage
| Autor: | Helen M. North, Audrey Macquet, Jocelyne Kronenberger, Marie-Christine Ralet, Annie Marion-Poll |
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| Přispěvatelé: | Biologie des Semences (LBS), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
0106 biological sciences
food.ingredient Pectin Physiology Arabidopsis Carbohydrates Plant Science Biology 01 natural sciences Antibodies 03 medical and health sciences chemistry.chemical_compound food PECTIN Adhesives [SDV.IDA]Life Sciences [q-bio]/Food engineering [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Cellulose 030304 developmental biology 0303 health sciences Staining and Labeling Cell Biology General Medicine Galactan RHAMNOGALACTURONAN Plant Mucilage chemistry Mucilage Biochemistry Covalent bond Seeds CELLULOSE Imbibition Layer (electronics) 010606 plant biology & botany SEED MUCILAGE |
| Zdroj: | Plant and Cell Physiology Plant and Cell Physiology, Oxford University Press (OUP), 2007, 48 (7), pp.984-999. ⟨10.1093/pcp/pcm068⟩ |
| ISSN: | 0032-0781 1471-9053 |
| Popis: | International audience; A comprehensive analysis was carried out of the composition of seed coat mucilage from Arabidopsis thaliana using the Columbia- 0 accession. Pectinaceous mucilage is released from myxospermous seeds upon imbibition, and in Arabidopsis consists of a water- soluble, outer layer and an adherent, inner layer. Analysis of monosaccharide composition in conjunction with digestion with pectolytic enzymes conclusively demonstrated that the principal pectic domain of both layers was rhamnogalacturonan I, and that in the outer layer this was unbranched. The macromolecular characteristics of the water- soluble mucilage indicated that the rhamnogalacturonan molecules in the outer layer were in a slightly expanded random- coil conformation. The inner, adherent layer remained attached to the seed, even after extraction with acid and alkali, suggesting that its integrity was maintained by covalent bonds. Confocal microscopy and monosaccharide composition analyses showed that the inner layer! can be separated into two domains. The internal domain contained cellulose microfibrils, which could form a matrix with RGI and bind it to the seed. In effect, in the mum5- 1 mutant where most of the inner and outer mucilage layers were water soluble, cellulose remained attached to the seed coat. Immunolabeling with anti- pectin antibodies indicated the presence of galactan and arabinan in the inner layer, with the latter only present in the non- cellulose- containing external domain. In addition, JIM5 and JIM7 antibodies labeled different domains of the inner layer, suggesting the presence of stretches of homogalacturonan with different levels of methyl esterification. |
| Databáze: | OpenAIRE |
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