Kinetics of antibody-secreting cell and fecal IgA responses after oral cholera vaccination in different age groups in a cholera endemic country
| Autor: | Arifuzzaman Khan, Sarmin Akter, Ann-Mari Svennerholm, Tanzeem Ahmed Rafique, Firdausi Qadri, Marjahan Akhtar, Laila N. Islam, Anna Lundgren, Amit Saha, Taufiqur Rahman Bhuiyan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Time Factors Adolescent Administration Oral medicine.disease_cause 03 medical and health sciences Feces Young Adult 0302 clinical medicine Immune system Medicine Humans 030212 general & internal medicine Antibody-Producing Cells Vibrio cholerae Bangladesh General Veterinary General Immunology and Microbiology biology business.industry Cholera toxin Public Health Environmental and Occupational Health Infant Cholera Vaccines Middle Aged medicine.disease Cholera Antibodies Bacterial 3. Good health Immunoglobulin A Vaccination 030104 developmental biology Infectious Diseases Child Preschool Immunoglobulin G Immunology biology.protein Molecular Medicine Female Antibody business Cholera vaccine Breast feeding |
| Zdroj: | Vaccine. 35(2) |
| ISSN: | 1873-2518 |
| Popis: | Immune responses to oral enteric vaccines in children and infants may be influenced by factors such as age, previous priming with related microorganisms and breast feeding. In this study, we aimed to determine optimal time points to assess immune responses to oral enteric vaccines in different clinical specimens. This was done by investigating antibody secreting cell (ASC) and fecal antibody responses on different days after vaccination using the licensed oral cholera vaccine Dukoral, containing cholera toxin B-subunit (rCTB) and inactivated Vibrio cholerae bacteria, as a model vaccine. Two vaccine doses were given 2weeks apart to infants (6-11months), young children (12-18months), toddlers (19months-5years) and adults in a cholera endemic country (Bangladesh). IgA ASC responses, as determined by the antibodies in lymphocyte supernatant (ALS) assay, plasma IgA and IgG responses and secretory IgA (SIgA) responses in extracts of fecal samples were evaluated 4/5 and 7days after each vaccination. After the first vaccine dose, anti-CTB ALS IgA responses in adults and toddlers were high and comparable on day 5 and 7, while responses were low and infrequent in young children. After the second dose, highest ALS responses were detected on day 5 among the time points studied in all age groups and the responses declined until day 7. In contrast, plasma IgA and IgG anti-CTB responses were high both on day 5 and 7 after the second dose. Fecal SIgA responses in young children and infants were highest on day 7 after the second dose. Our results suggest that ASC/ALS responses to two doses of the oral cholera vaccine Dukoral and related oral vaccines should be analyzed earlier than previously recommended (day 7) at all ages. Fecal antibody responses should preferably be analyzed later than ASC/ALS responses to detect the highest antibody responses. |
| Databáze: | OpenAIRE |
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