The Relevance of a Novel Quantitative Assay to Detect up to 40 Major Streptococcus pneumoniae Serotypes Directly in Clinical Nasopharyngeal and Blood Specimens
| Autor: | Thomas Bénet, Werner C. Albrich, Melina Messaoudi, Patrícia Costa, Mariam Sylla, Gláucia Paranhos-Baccalà, Mark van der Linden, Monidarin Chou, Milen Milenkov, Hubert P. Endtz, Nathalie Richard, Jean-Noel Telles, Keith P. Klugman |
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| Přispěvatelé: | Laboratoire des pathogènes émergents -- Emerging Pathogens Laboratory (LPE-Fondation Mérieux), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Infectious Diseases and Hospital Epidemiology [Zurich], University hospital of Zurich [Zurich], University of the Witwatersrand [Johannesburg] (WITS), RWTH-Aachen University Hospital, Rheinisch-Westfälische Technische Hochschule Aachen University (RWTH), Service d'Hygiène, Epidémiologie et Prévention [Hôpital Edouard Herriot - HCL], Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), University of Health Sciences [Phnom-Penh, Cambodia] (UHS), Gabriel Toure Hospital [Bamako, Mali], Fiocruz Paraná - Instituto Carlos Chagas / Carlos Chagas Institute [Curitiba, Brésil] (ICC), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Réanimation Pédiatrique Hôpital Femme Mère Enfant, hospices civils de Lyon, Emory University School of Medicine, Emory University [Atlanta, GA], Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center [Rotterdam] (Erasmus MC), Medical Microbiology & Infectious Diseases, Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Rheinisch-Westfälische Technische Hochschule Aachen (RWTH), Fundação Oswaldo Cruz (FIOCRUZ) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Serotype Pulmonology Physiology Epidemiology lcsh:Medicine Artificial Gene Amplification and Extension medicine.disease_cause Pathology and Laboratory Medicine Mali Polymerase Chain Reaction Pediatrics law.invention Cohort Studies Families South Africa law Nasopharynx Medicine and Health Sciences Multiplex lcsh:Science Child Children Polymerase chain reaction Multidisciplinary Streptococcus Bacterial Hematology 3. Good health Body Fluids Bacterial Pathogens Pneumococcal infections Blood Infectious Diseases Streptococcus pneumoniae Medical Microbiology Child Preschool [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.IMM]Life Sciences [q-bio]/Immunology France Anatomy Pathogens Cambodia Brazil Research Article DNA Bacterial Adult 030106 microbiology Biology Research and Analysis Methods Serogroup Microbiology Infectious Disease Epidemiology Pneumococcal Infections 03 medical and health sciences Species Specificity Multiplex polymerase chain reaction medicine Adults Humans Serotyping Molecular Biology Techniques Preschool Molecular Biology Microbial Pathogens lcsh:R Biology and Life Sciences Reproducibility of Results Pneumonia DNA medicine.disease Virology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Age Groups People and Places Population Groupings lcsh:Q Multiplex Polymerase Chain Reaction |
| Zdroj: | PLoS ONE PLoS ONE, 2016, 11 (3), pp.e0151428. ⟨10.1371/journal.pone.0151428⟩ PLoS One (print), 11(3). Public Library of Science PLoS ONE, Public Library of Science, 2016, 11 (3), pp.e0151428. ⟨10.1371/journal.pone.0151428⟩ PLoS ONE, Vol 11, Iss 3, p e0151428 (2016) PLOS ONE 11(3), e0151428 (2016). doi:10.1371/journal.pone.0151428 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0151428⟩ |
| Popis: | International audience; For epidemiological and surveillance purposes, it is relevant to monitor the distribution and dynamics of Streptococcus pneumoniae serotypes. Conventional serotyping methods do not provide rapid or quantitative information on serotype loads. Quantitative serotyping may enable prediction of the invasiveness of a specific serotype compared to other serotypes carried. Here, we describe a novel, rapid multiplex real-time PCR assay for identification and quantification of the 40 most prevalent pneumococcal serotypes and the assay impacts in pneumonia specimens from emerging and developing countries. Eleven multiplex PCR to detect 40 serotypes or serogroups were optimized. Quantification was enabled by reference to standard dilutions of known bacterial load. Performance of the assay was evaluated to specifically type and quantify S. pneumoniae in nasopharyngeal and blood samples from adult and pediatric patients hospitalized with pneumonia (n = 664) from five different countries. Serogroup 6 was widely represented in nasopharyngeal specimens from all five cohorts. The most frequent serotypes in the French, South African, and Brazilian cohorts were 1 and 7A/F, 3 and 19F, and 14, respectively. When both samples were available, the serotype in blood was always present as carriage with other serotypes in the nasopharynx. Moreover, the ability of a serotype to invade the bloodstream may be linked to its nasopharyngeal load. The mean nasopharyngeal concentration of the serotypes that moved to the blood was 3 log-fold higher than the ones only found in the nasopharynx. This novel, rapid, quantitative assay may potentially predict some of the S. pneumoniae serotypes invasiveness and assessment of pneumococcal serotype distribution. |
| Databáze: | OpenAIRE |
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