Studying the genotoxic effects induced by two kinds of bentonite particles on human B lymphoblast cells in vitro
| Autor: | Mingluan Xing, Jiliang He, Meibian Zhang, Xing-lin Fang, Xiaoxue Li, Qing Chen, Yezhen Lu |
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| Rok vydání: | 2011 |
| Předmět: |
DNA damage
Health Toxicology and Mutagenesis Biology urologic and male genital diseases medicine.disease_cause Cell Line Genetics medicine Humans B-Lymphocytes Micronucleus Tests Dose-Response Relationship Drug Lymphoblast Molecular biology In vitro Comet assay Solubility Toxicity Micronucleus test Bentonite Comet Assay Micronucleus hormones hormone substitutes and hormone antagonists Genotoxicity DNA Damage |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 720:62-66 |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/j.mrgentox.2010.12.009 |
| Popis: | The aim of the present study was to evaluate the genotoxic effects induced by native and active bentonite particles (BPs) on human B lymphoblast cells using comet assay and cytokinesis-block micronucleus (CBMN) assay in vitro. The cells were exposed to BPs at the concentrations of 30, 60, 120 and 240μg/ml for 24, 48 and 72h, respectively. The quartz contents of native and active BPs were 6.80±0.20 and 6.50±0.10%, respectively. Gypsum and DQ-12 quartz served as negative and positive controls. The results of comet assay showed that DNA damage induced by native and active BPs was significantly higher than that induced by gypsum control (P0.05 or0.01), and increased with exposure concentration and duration. When the cells were exposed to BPs at the doses of 120 and 240μg/ml for 72h, DNA damage induced by active BPs and native BPs was significantly higher than that induced by DQ-12 quartz (P0.01), and DNA damage induced by active BPs enhanced significantly, as compared with native BPs (P0.01). The results of CBMN assay demonstrated that both native BPs and active BPs could induce significant micronuclei, as compared with gypsum control (P0.05 or0.01). However, there was no significant difference of micronucleus frequency (MNF) among native BPs, active BPs and DQ-12 quartz. The water-soluble fractions from two kinds of BPs did not induce significant DNA damage and micronuclei. These findings indicated that the genotoxicity induced by active BPs and native BPs could be detected in comet assay and CBMN assay in vitro, the insoluble particle fractions from BPs may play a main role in the genotoxic effects induced by BPs. |
| Databáze: | OpenAIRE |
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