Phenotypicgenotypic study of antimicrobial profile of bacteria isolates from environmental samples

Autor: Indrani Karunasagar, Dechamma Muthappa Mundanda, D.S. Akhila, Anirban Chakraborty, Rajeshwari Vittal, Vijaya Kumar Deekshit, Santhosh Kogaluru Shivakumaraswamy, Juliet Roshini Mohan Raj
Rok vydání: 2019
Předmět:
0301 basic medicine
Veterinary medicine
medicine.drug_class
030106 microbiology
Antibiotics
lcsh:Medicine
India
Microbial Sensitivity Tests
medicine.disease_cause
Antimicrobial resistance
General Biochemistry
Genetics and Molecular Biology
genotypic characterization
03 medical and health sciences
0302 clinical medicine
Antibiotic resistance
multidrug resistance
Ampicillin
Drug Resistance
Bacterial
Gram-Negative Bacteria
medicine
Environmental Microbiology
Escherichia coli
environmental samples
Humans
030212 general & internal medicine
Citrobacter
biology
lcsh:R
Pathogenic bacteria
General Medicine
biology.organism_classification
Antimicrobial
Anti-Bacterial Agents
Multiple drug resistance
Antimicrobial resistance - environmental samples - genotypic characterization - multidrug resistance
Original Article
Bacteria
medicine.drug
Zdroj: The Indian Journal of Medical Research
Indian Journal of Medical Research, Vol 149, Iss 2, Pp 232-239 (2019)
ISSN: 0971-5916
Popis: Background & objectives: The resistance to antibiotics in pathogenic bacteria has increased at an alarming rate in recent years due to the indiscriminate use of antibiotics in healthcare, livestock and aquaculture. In this context, it is necessary to monitor the antibiotic resistance patterns of bacteria isolated from the environmental samples. This study was conducted to determine the phenotypic and genotypic profile of antimicrobial resistance in Gram-negative bacteria isolated from environmental samples. Methods: Two hundred and fifty samples were collected from different sources, viz. fish and fishery products (99), livestock wastes (81) and aquaculture systems (70), in and around Mangaluru, India. Isolation, identification and antimicrobial profiling were carried out as per standard protocols. The isolates were screened for the presence of resistance genes using PCR. Results: A total of 519 Gram-negative bacteria comprising Escherichia coli (116), Salmonella spp. (14), Vibrio spp. (258), Pseudomonas spp. (56), Citrobacter spp. (26) and Proteus spp. (49) were isolated and characterized from 250 samples obtained from different sources. A total of 12 antibiotics were checked for their effectiveness against the isolates. While 31.6 per cent of the isolates were sensitive to all the antibiotics used, 68.4 per cent of the isolates showed resistance to at least one of the antibiotics used. One-third of the isolates showed multidrug resistance. Maximum resistance was observed for ampicillin (43.4%), followed by nitrofurantoin (20.8%). Least resistance was seen for carbapenems and chloramphenicol. PCR profiling of the resistant isolates confirmed the presence of resistance genes corresponding to their antibiotic profile. Interpretation & conclusions: This study results showed high rate of occurrence of antimicrobial resistance and their determinants in Gram-negative bacteria isolated from different environmental sources.
Databáze: OpenAIRE
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