Cocaine- and amphetamine-regulated transcript (CART) peptide in the enteric nervous system of the porcine esophagus
| Autor: | Sławomir Gonkowski, Krystyna Makowska, Piotr Lech, Adam Osowski, Ewa Kruminis-Kaszkiel, Liliana Rytel |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cart medicine.medical_specialty Swine Vasoactive intestinal peptide Myenteric Plexus Nerve Tissue Proteins Biology General Biochemistry Genetics and Molecular Biology Cocaine and amphetamine regulated transcript Enteric Nervous System 03 medical and health sciences 0302 clinical medicine Esophagus Internal medicine mental disorders parasitic diseases medicine Submucous plexus Animals Galanin Myenteric plexus Neurons General Immunology and Microbiology virus diseases General Medicine Submucous Plexus 030104 developmental biology medicine.anatomical_structure Endocrinology nervous system Peripheral nervous system Enteric nervous system Female General Agricultural and Biological Sciences hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery |
| Zdroj: | Comptes rendus biologies. 341(6) |
| ISSN: | 1768-3238 |
| Popis: | Cocaine- and amphetamine-regulated transcript peptide (CART) is widely distributed within the central and peripheral nervous system. In the brain, CART is considered as the main anorectic peptide involved in the regulation of food intake. Contrary to the central nervous system, a lot of aspects connected with the distribution and functions of CART within the enteric nervous system (ENS) still remain unknown. The aim of the present study was to investigate, for the first time, the population of CART-like immunoreactive (CART-LI) neurons within the porcine esophagus and the denotation of their neurochemical coding. During this experiment, the distribution of CART-LI neurons and the colocalization of CART with other neuronal active substances were examined using standard double- and triple-immunofluorescence techniques in enteric plexuses of cervical, thoracic, and abdominal esophagus fragments. The obtained results showed that CART is present in a relatively high percentage of esophageal neurons (values fluctuated from 45.2±0.9% in the submucous plexus of the thoracic esophagus to 58.1±5.0% in the myenteric plexus of the same fragment of the esophagus). Moreover, CART colocalized with a wide range of other active neuronal substances, mainly with the vesicular acetylcholine transporter (VAChT, a marker of cholinergic neurons), neuronal isoform of nitric oxide synthase (nNOS, a marker of nitrergic neurons), vasoactive intestinal polypeptide (VIP) and galanin (GAL). The number of CART-positive neuronal cells and their neurochemical coding clearly depended on the fragment of esophagus studied and the type of enteric plexus. The obtained results suggest that CART may play important and multidirectional roles in the neuronal regulation of esophageal functions. |
| Databáze: | OpenAIRE |
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