Amyloid-Beta Induces Different Expression Pattern of Tissue Transglutaminase and Its Isoforms on Olfactory Ensheathing Cells: Modulatory Effect of Indicaxanthin

Autor: Agatina Campisi, Giovanni Sposito, Rosalia Pellitteri, Mario Allegra, Michela Spatuzza, Giuseppina Raciti, Alessandro Attanzio, Rosaria Grasso, Luisa Tesoriere, Ugo Chiacchio, Maria A. Chiacchio
Přispěvatelé: Campisi A., Raciti G., Sposito G., Grasso R., Chiacchio M.A., Spatuzza M., Attanzio A., Chiacchio U., Tesoriere L., Allegra M., Pellitteri R.
Rok vydání: 2021
Předmět:
Pyridines
tissue transglutaminase
olfactory ensheathing cells
amyloid-beta
oxidative stress
Indicaxanthin
self-renewal
Apoptosis
Amyloid‐beta
Vimentin
lcsh:Chemistry
Nestin
Mice
chemistry.chemical_compound
Protein Isoforms
Cyclin D1
lcsh:QH301-705.5
Spectroscopy
biology
Superoxide
Opuntia
Cell Differentiation
General Medicine
Olfactory Bulb
Betaxanthins
Computer Science Applications
Cell biology
Amyloid beta
Tissue transglutaminase
Olfactory Ensheathing Cells
Amyloid-Beta
In-dicaxanthin
Article
Gene Expression Regulation
Enzymologic
Catalysis
Inorganic Chemistry
Alzheimer Disease
GTP-Binding Proteins
Glial Fibrillary Acidic Protein
Animals
Humans
Protein Glutamine gamma Glutamyltransferase 2
Viability assay
Physical and Theoretical Chemistry
Molecular Biology
Amyloid beta-Peptides
Transglutaminases
Organic Chemistry
Self‐renewal
Nerve Regeneration
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Oxidative stress
Olfactory ensheathing cells
biology.protein
Olfactory ensheathing glia
Reactive Oxygen Species
Zdroj: International journal of molecular sciences (Online) 22 (2021). doi:10.3390/ijms22073388
info:cnr-pdr/source/autori:Agata Campisi; Giuseppina Raciti ; Giovanni Sposito; Rosaria Grasso; Maria A. Chiacchio; Michela Spatuzza; Alessandro Attanzio; Ugo Chiacchio; Luisa Tesoriere; Mario Allegra; and Rosalia Pellitteri/titolo:Amyloid-Beta Induces Different Expression Pattern of Tissue Transglutaminase and Its Isoforms on Olfactory Ensheathing Cells: Modulatory Effect of Indicaxanthin/doi:10.3390%2Fijms22073388/rivista:International journal of molecular sciences (Online)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:22
International Journal of Molecular Sciences
Volume 22
Issue 7
International Journal of Molecular Sciences, Vol 22, Iss 3388, p 3388 (2021)
ISSN: 1422-0067
Popis: Alzhèimer Disease (AD) is characterized by protein aggregates in the brain, including amyloid-beta (Aβ), a substrate for tissue transglutaminase (TG2). We assessed the effect of full native peptide of Aβ (1–42), the fragments (25–35 and 35–25) on TG2 expression and its isoforms (Long and Short) on mouse Olfactory Ensheathing Cells (OECs). The levels of cytoskeletal proteins, Vimentin and Glial Fibrillary Acid Protein, were also studied. The effect of the pre-treatment with Indicaxanthin on cell viability, total Reactive Oxygen Species, superoxide anion and apoptotic pathway activation was assessed. Since Nestin is co-expressed in pluripotent stem cells with cyclin D1, their levels were also evaluated. Our findings highlight that Aβ (1–42) and its fragments induced an increase of TG2 levels and the different expression pattern of its isoforms on OECs. Indicaxanthin pre-treatment was able to reduce TG2 over-expression upregulated by Aβ exposure of cells differently modulating its isoforms. In addition, it prevented total Reactive Oxygen Species and superoxide anion production, it reduced Glial Fibrillary Acid Protein and Vimentin levels and inhibited apoptotic pathway activation. It also leaded to an increase of Nestin and cyclin D1 expression, stimulating stem cells renewal through the reparative activity played by TG2. Our results suggest that Aβ in OECs, both in the absence and in the presence of Indicaxanthin, might differently induce the transition of TG2 between “closed” and “open” conformation, providing a new mechanism involved in the signal pathways activated by the protein in Aβ injury important for neural regeneration of AD.
Databáze: OpenAIRE
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